Abstract
Protoplasts isolated from lateral shoot buds of cotyledon-free pea embryo axes were regenerated to callus. Protoplast derived calluses with a diameter of about 1cm were transferred to shoot induction media, containing different concentrations (1–50µM) of thidiazuron. Shoot formation was observed after 16 weeks up to 12% efficiency. Thidiazuron (10µM) was the most effective concentration in all experiments. Shoot buds elongated in medium supplemented with N-isopentenyl adenine and indole-3-butyric acid. Since rooting was almost impossible in these thidiazuron-induced shoots, shoots were grafted onto young pea seedlings and regenerated to fertile plants.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- 2ip:
-
N-isopentenyl adenine
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- MES:
-
2-(Nmorpholino)ethane acid
- NAA:
-
naphthaleneacetic acid
- TDZ:
-
thidiazuron
- BAP:
-
6-benzylaminopurine
- PEG:
-
polyethylene glycol
References
Capelle SC, Mok DWS, Kirchner SC, Mok MC (1983) Plant Physiol. 73:796–802.
Cardi T, Adamo F, Fillipone E (1991) J. Genet.&Breed. 45:67–70.
Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell Res. 50:151–158.
Gamborg OL, Constabel F, Shyluk JP (1974) Physiol. Plant. 30:143–148.
Gill R, Saxena PK (1992) Can. J. Bot. 70:1186–1192.
Hussey G, Gunn HV (1984) Plant Sci. Lett. 37:143–148.
Jackson JA, Hobbs SLA (1990) In Vitro Cell. Dev. Biol. 26:835–838.
Kublakova M, Tejklova E, Griga M (1988) Biol. Plantarum (Praha) 30:179–184.
Kysely W, Myers JR, Lazzeri PA, Collins GB, Jacobsen HJ (1887) Plant Cell Rep. 6:305–308.
Lehminger-Mertens R, Jacobsen H-J (1989a) In Vitro Cell. Dev. Biol. 6:571–574.
Lehminger-Mertens R, Jacobsen H-J (1989b) Plant Cell Rep. 8:379–382.
Leshem B, Roner R, Lurie S (1994) J. Plant Physiol. 143:344–348.
Lu CY (1993) In Vitro Cell. Dev. Biol. 29P:92–96.
Malik KA, Saxena PK (1992) Planta 186: 384–389.
Malmberg RL (1979) Planta 146:243–244.
Mok MC, Mok DWS, Armstrong DJ, Shudo K, Isogai Y, Okamoto T (1982) Phytochem. 21:1509–1511.
Mroginski LA, Kartha KK (1981) Plant Cell Rep. 1:64–66.
Murashige T, Skoog F (1962) Physiol. Plant. 15:473–497.
Natali L, Cavallini A (1987) Plant Breeding 99:172–176.
Nauerby B, Madsen M, Christiansen J, Wyndaele R (1991) Plant Cell Rep. 9:676–679.
Özcan S, Barghchi M, Firek S, Draper J (1992) Plant Cell Rep. 11:44–47.
Özcan S, Barghchi M, Firek S, Draper J (1993) Plant Cell, Tissue and Organ Culture 34:271–277.
Pickardt T, Schieder O, Saalbach I, Tegeder M, Machemehl F, Saalbach G, Kohn H, Müntz K (1992) Plant Tissue Culture and Gene Manipulation for Breeding and Formation of Phytochemicals/ Japan 89–94.
Preece JE, Imel MR (1991) Scient. Horti. 48:159–170.
Puonti-Kaerlas J, Eriksson T (1988) Plant Cell Rep. 7:242–245.
Puonti-Kaerlas J, Ottosson A, Eriksson T (1992) Plant Cell, Tissue and Organ Culture 30:141–148.
Rubluo A, Kartha KK, Mroginski LA, Dyck J (1984) J. Plant Physiol. 117:119–130.
Sriskandarajah S, Skirvin RM, Abu-Qaoud H, Korban SS (1990) J. Hort. Science 65(2):113–121.
Tetu T, Sangwan RS, Sangwan-Norreel BS (1990) J. Plant Physiol. 137: 102–109.
Thomas JC, Katterman FR (1986) Plant Physiol. 81:681–683.
Author information
Authors and Affiliations
Additional information
Communicated by H. Lörz
Rights and permissions
About this article
Cite this article
Böhmer, P., Meyer, B. & Jacobsen, HJ. Thidiazuron-induced high frequency of shoot induction and plant regeneration in protoplast derived pea callus. Plant Cell Reports 15, 26–29 (1995). https://doi.org/10.1007/BF01690247
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01690247