Summary
A regeneration system based on repetitive somatic embryogenesis was developed for peanut (Arachis hypogaea L.). Embryogenic suspension cultures were initiated using individual somatic embryos induced from immature cotyledons cultured on a modified Murashige and Skoog medium containing 40 mg/l 2,4-D for 30 days. After transfer to a modified MS liquid medium, the somatic embryos produced masses of secondary and tertiary embryos which continued to proliferate following manual separation and subculture of the embryogenic clumps. The cultures exhibited exponential growth, and have been maintained for over one year without apparent loss of embryogenic potential. Further embryo development, germination, and conversion were achieved by placing embryo clumps onto hormone-free, solid medium. The inclusion of a desiccation period during embryo development enhanced conversion four-fold. Plants have been established in soil and appear to be phenotypically normal.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
1-naphthaleneacetic acid
- BA:
-
6-benzylaminopurine
- MSO:
-
Modified Murashige and Skoog basal medium
- EM:
-
embryogenic masses
Literature Cited
Baker CM, Wetzstein HY (1991) Pl Cell Rep In press
Finer JJ, McMullen MD (1990) Pl Cell Rep 8: 586–589
Finer JJ, Nagasawa A (1988) Pl Cell Tiss Organ Cult 15: 125–136
Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50: 150–158
Gordon-Kamm WJ, Spencer TM, Mangano ML, Adams TR, Daines RJ, Start WG, O'Brien JV, Chambers SA, Adams WR, Jr., Willetts NG, Rice TB, Mackey CJ, Krueger RW, Kausch AP, Lemaux PG (1990) Pl Cell 2: 603–618
Gray DJ (1989) In Vitro Cell Dev Biol 25: 1173–1178
Hazra S, Sathaye SS, Mascarenhas AF (1989) Bio/Tech 7: 949–951
Hidaka T, Omura M, Ugaki M, Tomiyama M, Kalo A, Ohshima M, Motoyoshi F (1990) Japan J Breed 40: 199–207
McGranahan GH, Leslie CA, Uratsu S, Martin LA, Dandekar AM (1988) Bio/Tech 6: 800–804
McMullen MD, Finer JJ (1991) J Cell Biochem Supplement 14E: 285
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Ozias-Akins P (1989) Pl Cell Rep 8: 217–218
Parrott WA, Dryden G, Vogt S, Hildebrand DF, Collins GB, Williams EG (1988) In Vitro Cell Dev Biol 24: 817–820
Parrott WA, Bailey MA, Durham RE, Mathews VH (1991a) In: Moss, JP (ed) Proceedings of the international workshop on biotechnology and plant improvement in Asia. ICRISAT Press, Hyderabad, In Press
Parrott WA, Merkle SA, Williams EG (1991b) In: Murray, DR (ed) Advanced methods in plant breeding and biotechnology. CAB International, Wallingford, pp 158–200
Sellars RM, Southward GM, Phillips GC (1990) Crop Sci 30:408–414
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Communicated by J. M. Widholm
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Durham, R.E., Parrott, W.A. Repetitive somatic embryogenesis from peanut cultures in liquid medium. Plant Cell Reports 11, 122–125 (1992). https://doi.org/10.1007/BF00232163
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DOI: https://doi.org/10.1007/BF00232163