Abstract
An efficient micropropagation system for Hylotelephium tatarinowii (Maxim.) H. Ohba, a rare medicinal plant, has been developed. Callus induced from leaf explants placed onto Murashige and Skoog (MS) medium with supplementation of plant growth regulators. When the concentration of 2,4-dicholorophenoxy acetic acid was as high as 2.0 mg l−1 in combination with 0.5 mg l−1 6-benzylaminopurine (6-BAP), the callus induction rate reached 92.1%. Adventitious shoots were observed on callus exposed to 1.0 mg l−1 6-BAP, with 81.5% frequency of shoot regeneration after 30 d. Flower buds appeared after subculture. Regenerated shoots could flower normally in vitro. Up to 100% of the regenerated shoots formed complete plantlets on half-strength MS medium without any growth regulator, with an average of 5.9 roots per shoot explant. Quantitative analysis of flavonoids and rutin showed that the phytochemical profile of callus and regenerated plants was similar to that of wild plants.
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Acknowledgments
This work was financially supported by the National Basic Research Program of China (973 Program, 2009CB522300), the Fund of State Key Laboratory of Phytochemistry and Plant Resources in West China (09708211Z1), Program of State Ethnic Affairs Commission (09ZY09), the “985” Project (MUC985), “111” Project (B08044), the Research Project of Minzu University of China (MUC0910KYZY46), and “211” Project (MUC211).
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Editor: N. J. Taylor
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Wang, J., Wang, J., Liu, K. et al. An efficient plant regeneration system with in vitro flavonoid accumulation for Hylotelephium tatarinowii (Maxim.) H. Ohba. In Vitro Cell.Dev.Biol.-Plant 46, 445–450 (2010). https://doi.org/10.1007/s11627-010-9315-6
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DOI: https://doi.org/10.1007/s11627-010-9315-6