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Effect of different plant growth regulators on micro-tuber induction and plant regeneration of Pinellia ternate (Thunb) Briet

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Abstract

An efficient micropropagation system for Pinellia ternate (Thunb) Briet, a traditional Chinese medicinal plant, has been developed. Petiole and lamina of P. ternate were used as explants and cultured on Murashige and Skoog (MS) medium containing different concentrations of different plant growth regulators. The results indicated that low concentration of 2,4-dicholorophenoxy acetic acid (2,4-D), indole-3-acetic acid (IAA) and α-naphthalene acetic acid (NAA) were suitable for micro-tuber induction, but callus induction rate increased with increasing concentrations of growth regulators. Tubers induction rates of petiole and leaf were (81.8 %–100 %) and (89.4 %–96.0 %) respectively, when 0.2 mg l−1 2, 4-dicholorophenoxy acetic acid, indole-3-acetic acid or α-naphthalene acetic acid were present in the medium. Tubers induction rates of petiole and leaf cultured on MS medium supplemented with 0.2–0.5 mg l−1 6-benzyl amino purine (6-BAP) were (94.1 %–100 %) and (96.0 %–100 %) respectively. When the concentration of 2,4-dicholorophenoxy acetic acid, α-naphthalene acetic acid and 6-benzyl amino purine was increased to 2.0 mg l−1, callus induction rates of petiole and leaf were 100 % and 98.2 %, 91.0 % and 36.0 %, 62.3 % and 70.0 %, respectively. Different concentration of kinetin (KT) and zeatin (ZT) had no significant effect on micro-tuber induction of petiole. Most petioles showed polarity during the cultivation of explants, when supplemented with different concentrations of auxin or cytokinin in the MS medium.

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Authors and Affiliations

  1. College of Life and Environment Sciences, Minzu University of China, Beijing, 100081, People’s Republic of China

    Junli Wang, Qian Wang, Jue Wang, Yuan Lu, Xuan Xiao & Weizhen Gong

  2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming, 650204, People’s Republic of China

    Jikai Liu

  3. College of Life and Environment Sciences, Minzu University of China, Zhong Guan Cun South Street 27, Beijing, 100081, People’s Republic of China

    Junli Wang

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  1. Junli Wang
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  2. Qian Wang
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  3. Jue Wang
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  4. Yuan Lu
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  5. Xuan Xiao
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  6. Weizhen Gong
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  7. Jikai Liu
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Correspondence to Junli Wang.

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Wang, J., Wang, Q., Wang, J. et al. Effect of different plant growth regulators on micro-tuber induction and plant regeneration of Pinellia ternate (Thunb) Briet. Physiol Mol Biol Plants 15, 359–365 (2009). https://doi.org/10.1007/s12298-009-0040-8

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