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High-frequency embryogenesis and regeneration of plants with high content of gentiopicroside from the Chinese medicinal plant Gentiana straminea Maxim.

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Abstract

Gentiana straminea Maxim. (MahuaQinjiao) is an important Chinese medicinal herb that is rich in secoiridoids. To increase the production of this plant, we have developed an efficient tissue culture system for high-frequency plant regeneration. We have also obtained plants with high secoiridoid monomer production through tissue culture and somatic embryogenesis. We have found that the MB medium is better than the B5 medium for callus induction. Calluses induced from immature seeds are superior to those from hypocotyls or young leaves in regeneration via somatic embryogenesis. We have also demonstrated that 2,4-dichlorophenoxyacetic acid is efficient for both callus induction and embryogenesis, indole-3-acetic acid is suitable for embryogenic callus proliferation, and N6-(benzyl)-adenine promotes both embryo development and the accumulation of gentiopicroside in the cultures. Regenerated plants have been selected for high gentiopicroside content. One plant contains 5.82% of gentiopicroside, which is higher than the control plants (1.20–3.73%). The regenerated plants are genetically more stable than the calluses based on both cytological and random amplified polymorphic DNA analyses.

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Acknowledgments

This study was supported by the National Basic Research Program of China, No. 2007CB948203; the National Natural Science Foundation of China, Nos. 30470154 and 30771116; New Century Training Program Foundation for Talents by the Ministry of Education, No. NCET-05-0581; and the Doctor Station Foundation Fellowship Chinese Natural Education Ministry, No. 20050422015. The authors are grateful to Roberta Greenwood and Weichang Yu for editing this manuscript.

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Authors and Affiliations

  1. The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Shanda Nanlu 27#, Jinan, 250100, People’s Republic of China

    Yunfei Cai, Yanling Liu, Zhenhua Liu, Fengning Xiang & Guangmin Xia

  2. Department of Bioscience and Biotechnology, Shandong Institute of Education, Jinan, 250013, People’s Republic of China

    Yunfei Cai & Feng Zhang

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  1. Yunfei Cai
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  2. Yanling Liu
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  3. Zhenhua Liu
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  4. Feng Zhang
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  5. Fengning Xiang
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  6. Guangmin Xia
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Correspondence to Fengning Xiang or Guangmin Xia.

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Editor: Neftali Ochoa-Alejo

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Cai, Y., Liu, Y., Liu, Z. et al. High-frequency embryogenesis and regeneration of plants with high content of gentiopicroside from the Chinese medicinal plant Gentiana straminea Maxim.. In Vitro Cell.Dev.Biol.-Plant 45, 730–739 (2009). https://doi.org/10.1007/s11627-009-9225-7

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