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Somatic embryogenesis of Gentiana genus I. The effect of the preculture treatment and primary explant origin on somatic embryogenesis of Gentiana cruciata (L.), G. pannonica (Scop.), and G. tibetica (King)

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Abstract

Experiments were carried out on three selected species of Gentiana genus: Gentiana cruciata (L.), G. pannonica (Scop.), and G. tibetica (King). Using MS medium supplemented with 2,4-D and kinetin a somatic embryogenesis system of plant regeneration was developed. Induction and intensity of somatic embryogenesis as the effect of integration of the following factors were studied, specifically: seedling pre-treatment (with and without GA3 treatment), light condition (light versus the dark), and type of explant (root, cotyledon and hypocotyl). Numerous significant differences between studied factors were observed and statistically proved.

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Abbreviations

GA3 :

gibberellic acid

2,4-D:

dichlorophenoxyacetic acid

MS:

Murashige and Skoog medium

Kin:

kinetin

NAA:

naphtaleneacetic acid

6BA:

6-benzyladenine

References

  • Bicknell R.A. 1984. Seed propagation of Gentiana scabra. Proceeding of the International Plant Propagators Society, 34: 396–401.

    Google Scholar 

  • Fujimura T., Komamine A. 1975. Effects of various growth regulators on the embryogenesis in carrot cell suspension culture. Plant Sci. Letters 5: 359–364.

    Article  CAS  Google Scholar 

  • Grubisic D., Giba Z., Konjevic R. 1995. Seed gemination of Gentiana cruciata L. Glas. Inst. Bot. I Baste Univ, U Beogradu, 29: 93–100.

    Google Scholar 

  • Hosokawa K., Nakano M., Oikawa Y., Yamamura S. 1996. Adventitious shoot regeneration from leaf, stem and root explants of commercial cultivars of Gentiana. Plant Cell Reports, 15: 578–581.

    Article  CAS  Google Scholar 

  • Lamproye A., Crevecoeur M., Kevers C., Gaspar Th. 1987. Multiplication vegetative in vitro de Gentiana lutea et de Gentiana pneumonanthe. Med. Fac. Landbouww. Rijksuniv. Gent., 52: 1255–1257.

    Google Scholar 

  • Meng Y., Gao Y., Jia J. 1996. Plant regeneration from protoplasts isolated from callus of Gentiana crassicaulis. Plant Cell Reports, 16: 88–91.

    CAS  Google Scholar 

  • Mikuła A., Rybczyński J.J., Skierski J. 2001. Somatic embryogenesis of Gentiana genus. II. Characterization of embryogenic suspensions of G. pannonica (Scop.) originated from various seedling explants during three years of culture. (in press).

  • Mikuła A., Wesołowska M., Kapusta J., Skrzypczak L., Rybczyński J.J. 1996. Cytomorphological studies of somatic embryogenesis of Gentiana tibetica (King) and G. cruciata (L.). Acta Soc. Bot. Pol., 65: 47–51.

    Google Scholar 

  • Momčilović I., Grubišić D., Nešković M. 1997. Micropropagation of four Gentiana species (G. lutea, G. cruciata, G. purpurea and G. acaulis). Plant Cell Tissue and Organ Culture, 49: 141–144.

    Article  Google Scholar 

  • Morgan E.R., Butler M.R., Bicknell R.A. 1997. In vitro propagation of Gentiana cerina and Gentiana corymbifera. New Zeal. J. Crop Hort. Sci., 25: 1–8.

    Google Scholar 

  • Murashige T. 1964. Analysis of the inhibition of organ formation in tobacco tissue culture by gibberelin. Physiol. Plant., 17: 636–643.

    Article  CAS  Google Scholar 

  • Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15: 473–497.

    Article  CAS  Google Scholar 

  • Ørnstrup H., Mřlgaard J.P., Farestveit B. 1993. Somatic embryogenesis and plant regeneration from cell suspensions of Exacum affine. Plant Cell, Tissue and Organ Culture, 35: 37–41.

    Article  Google Scholar 

  • Starling R.J., Newbury G.L., Callow J.A. 1986. Putative auxin receptors in tabacco callus. in: L.A. Whithers, P.G. Alderson (Eds.), Plant Tissue Culture and Its Agricultural Applications, Butterworths, London, pp. 47–54.

    Google Scholar 

  • Tabira H. 1994. Development of micropropagation system of Gentiana triflora. Abstracts. of XXIVth International Horticultural Congress. 21–27 August, Kyoto, Japan., 186–187

  • Takahata Y., Jomori H. 1989. Plant regeneration from mesophyll protoplasts of gentiana (Gentiana scabra Bungei). Plant Tiss. Cult. Lett., 6: 19–21.

    Google Scholar 

  • Yuan Y.M., Küpfer P., Doyle J. 1996. Infrageneric phylogeny of the genus Gentiana (Gentianaceae) inferred from nucleotide sequences of the internal transcribed spacers (ITS) of nuclear ribosomal DNA. Am. J. Bot., 83: 641–652.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Botanical Garden - Centre For Biological Diversity Conservation, Polish Academy of Sciences, ul. Prawdziwka 2, 02-576, Warszawa, Poland

    Anna Mikuła & Jan J. Rybczyński

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  1. Anna Mikuła
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  2. Jan J. Rybczyński
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Correspondence to Anna Mikuła.

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Mikuła, A., Rybczyński, J.J. Somatic embryogenesis of Gentiana genus I. The effect of the preculture treatment and primary explant origin on somatic embryogenesis of Gentiana cruciata (L.), G. pannonica (Scop.), and G. tibetica (King). Acta Physiol Plant 23, 15–25 (2001). https://doi.org/10.1007/s11738-001-0017-x

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  • DOI: https://doi.org/10.1007/s11738-001-0017-x

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