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Response of plasma membrane H+-ATPase in rice (Oryza sativa) seedlings to simulated acid rain

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Abstract

Understanding the adaptation of plants to acid rain is important to find feasible approaches to alleviate such damage to plants. We studied effects of acid rain on plasma membrane H+-ATPase activity and transcription, intracellular H+, membrane permeability, photosynthetic efficiency, and relative growth rate during stress and recovery periods. Simulated acid rain at pH 5.5 did not affect plasma membrane H+-ATPase activity, intracellular H+, membrane permeability, photosynthetic efficiency, and relative growth rate. Plasma membrane H+-ATPase activity and transcription in leaves treated with acid rain at pH 3.5 was increased to maintain ion homeostasis by transporting excessive H+ out of cells. Then intracellular H+ was close to the control after a 5-day recovery, alleviating damage on membrane and sustaining photosynthetic efficiency and growth. Simulated acid rain at pH 2.5 inhibited plasma membrane H+-ATPase activity by decreasing the expression of H+-ATPase at transcription level, resulting in membrane damage and abnormal intracellular H+, and reduction in photosynthetic efficiency and relative growth rate. After a 5-day recovery, all parameters in leaves treated with pH 2.5 acid rain show alleviated damage, implying that the increased plasma membrane H+-ATPase activity and its high expression were involved in repairing process in acid rain-stressed plants. Our study suggests that plasma membrane H+-ATPase can play a role in adaptation to acid rain for rice seedlings.

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Acknowledgments

The authors are grateful for the financial support from the National Natural Science Foundation of China (31000245, 31370517).

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Chanjuan Liang

  2. School of Environmental and Civil Engineering, Jiangnan University, Wuxi, 214122, China

    Chanjuan Liang, Yuqing Ge, Lei Su & Jinjin Bu

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  1. Chanjuan Liang
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  2. Yuqing Ge
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Correspondence to Chanjuan Liang.

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Responsible editor: Elena Maestri

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Liang, C., Ge, Y., Su, L. et al. Response of plasma membrane H+-ATPase in rice (Oryza sativa) seedlings to simulated acid rain. Environ Sci Pollut Res 22, 535–545 (2015). https://doi.org/10.1007/s11356-014-3389-3

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  • DOI: https://doi.org/10.1007/s11356-014-3389-3

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