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Construction of a genetic linkage map and identification of molecular markers in peach rootstocks for response to peach tree short life syndrome

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Abstract

Peach tree short life (PTSL) is a devastating disease syndrome of peach [Prunus persica (L.) Batsch] caused by multiple factors; the molecular biology of its tolerance/susceptibility is unknown. The difficulty of studying PTSL is that tree survival or death is not obvious until 3 to 5 years after planting when the symptoms of PTSL first appear. Tolerance to PTSL was unknown in Prunus until the rootstock Guardian® ‘BY520-9’ was introduced into commercial orchards in 1994. To study the genetics of the response to PTSL, a controlled F2 cross was made between Guardian® ‘BY520-9’ selection 3-17-7 (PTSL-tolerant) and Nemaguard (PTSL-susceptible). An F1 hybrid was then selfed to generate an F2 population expected to segregate for PTSL response. One hundred fifty-one AFLPs and 21 SSRs, including anchor loci from the Prunus reference genetic map, were used to construct a molecular genetic map based on 100 F2 seedlings. This map covers a genetic distance of 737 cM with an average marker spacing of 4.7 cM and will be used as a framework to construct a highly saturated molecular genetic map. Of the 140 mapped AFLP markers, 38 were associated with PTSL response, as identified previously by bulked segregant analysis. The distribution of the markers associated with PTSL response on the newly constructed genetic map was compared with the recently published Prunus resistance map. This comparison revealed that some resistance gene analogs and several PTSL-associated AFLP markers were located in the same regions in several Prunus linkage groups: G1, G2, G4, G5, and G6. This peach rootstock map can also be viewed and compared with other Prunus maps in comparative map viewer CMap in the Genome Database for Rosaceae (GDR) at http://www.rosaceae.org

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Acknowledgment

The authors thank Dr. Bryon Sosinski (NC State University, USA) and Dr. Zhen-Xiang Lu (Lethbridge Research Centre, Agriculture and Agri-Food Canada) who made the original four F2 crosses designed to segregate for PTSL response (PTSL-tolerant Guardian® selections 3-17-7, 4-26-2, 6-39-7, and SL1089 crossed with the highly PTSL-susceptible Nemaguard). Dr. Marisa Badenes (Instituto Valenciano de Investigaciones Agrarias, Spain), Dr. Renate Horn (University of Rostock, Germany), Dr. Alexander Kozik (UC Davis, USA), and Dr. Tatyana Zhebentyayeva (Clemson University, USA) are acknowledged for reviewing the manuscript and/or useful comments. This article is technical contribution no. 5098 of the Clemson University Agricultural Experiment Station. The work was supported by the Cooperative State Research, Education, and Extension Service (CSREES)/USDA under project SC-1700005, CSREES special research grant “Peach Tree Short Life in South Carolina”, and Natural Resources Institute grant 98-35311-6719 “Molecular Genetic Studies of Ring Nematode Tolerance/Peach Tree Short Life Tolerance.”

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Authors and Affiliations

  1. Department of Horticulture, Clemson University, Clemson, SC, USA

    A. V. Blenda, G. L. Reighard & W. V. Baird

  2. Department of Genetics and Biochemistry, Clemson University, Clemson, SC, USA

    A. V. Blenda, L. L. Georgi, S. D. Forrest, M. Muñoz-Torres & A. G. Abbott

  3. Istituto Sperimentale per la Frutticoltura (CRA), Rome, Italy

    I. Verde

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  1. A. V. Blenda
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  2. I. Verde
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  3. L. L. Georgi
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  4. G. L. Reighard
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  5. S. D. Forrest
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  6. M. Muñoz-Torres
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  7. W. V. Baird
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  8. A. G. Abbott
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Correspondence to A. V. Blenda.

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Communicated by Peré Arús

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Blenda, A.V., Verde, I., Georgi, L.L. et al. Construction of a genetic linkage map and identification of molecular markers in peach rootstocks for response to peach tree short life syndrome. Tree Genetics & Genomes 3, 341–350 (2007). https://doi.org/10.1007/s11295-006-0074-9

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  • DOI: https://doi.org/10.1007/s11295-006-0074-9

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