Abstract
Different groups of biosurfactants exhibit diverse properties and display a variety of physiological functions in producer microorganisms; these include enhancing the solubility of hydrophobic/water-insoluble compound, heave metal binding, bacterial pathogenesis, cell adhesion and aggregation, quorum sensing and biofilm formation. Candida sphaerica was grown in a low cost medium, consisting of distilled water supplemented with 9% refinery residue of soybean oil and 9% corn steep liquor, for 144 h at 28°C and 150 rpm. The cell-free supernatant obtained at the end of the experiments was submitted to extraction, and afterward the biosurfactant was isolated using methanol with a yield of 9 g l−1. The critical micelle concentration of the biosurfactant was found to be 0.25 mg ml−1 with a surface tension of 25 mN m−1. Several concentrations of the biosurfactant (0.625–10 mg ml−1) were used to evaluate its antimicrobial and antiadhesive activities against a variety of microorganisms. The biosurfactant showed antimicrobial activity against Streptococcus oralis (68%), Candida albicans (57%), and Staphylococcus epidermidis(57.6%) for the highest concentration tested. Furthermore, the biosurfactant at a concentration of 10 mg ml−1 inhibited the adhesion between 80 and 92% of Pseudomonas aeruginosa, Streptococcus agalactiae, Streptococcus sanguis12. Inhibition of adhesion with percentages near 100% occurred for the higher concentrations of biosurfactant used. Results gathered in this study point to a potential use of the biosurfactant in biomedical applications.
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The authors acknowledge the funding agencies FACEPE, CNPq, FINEP, PRONEX, and UNICAMP for using their facilities and CAPES for sandwich doctorate scholarship.
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Luna, J.M., Rufino, R.D., Sarubbo, L.A. et al. Evaluation Antimicrobial and Antiadhesive Properties of the Biosurfactant Lunasan Produced by Candida sphaerica UCP 0995. Curr Microbiol 62, 1527–1534 (2011). https://doi.org/10.1007/s00284-011-9889-1
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DOI: https://doi.org/10.1007/s00284-011-9889-1