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Production and characterization of biosurfactant by marine bacterium Pseudomonas stutzeri (SSASM1)

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Abstract

Biosurfactant-producing bacteria were isolated from sediment sample collected from Pondicherry harbour region, south-east coast of India. Isolates were screened for the biosurfactant production using crude oil as substrate. The selected strain was identified by 16S rDNA gene sequencing analysis and was tentatively identified as Pseudomonas stutzeri (SSASM1) strain. Additionally, the emulsification activity was carried out and found 77.6%. The surface tension reduction test showed 33.5 mN/m. The biosurfactant produced was characterized using rhamnose test, Fourier-transform infrared spectroscopy and proton nuclear magnetic resonance (1H NMR) and found as rhamnolipid nature. The 1H NMR spectra of the biosurfactant displayed a number of signals confirming the presence of different sugar moieties and aromatic groups and also showed the occurrence of long aliphatic hydrocarbon chain (CH2). The 1HNMR graph emphasized the biosurfactant, showing the resonance signals between 0.792 and 1.845 ppm.

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Acknowledgements

The authors are thankful to Ministry of Earth Sciences (MoES), ICMAM-PD, for financial support through the scheme Seawater Quality Monitoring Programme under Coastal Research Scheme (Grant No. G4/11742/2012/ICMAMPD/SWQM/CASMB/35/2012). We also thank the authorities of Annamalai University for providing the necessary facilities during the entire study period.

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Authors and Affiliations

  1. Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, 608 502, Tamil Nadu, India

    S. Shekhar, A. Sundaramanickam, K. Saranya, M. Meena & S. Kumaresan

  2. Chettinad Hospital and Research Institute, Chettinad Health City IT Highway Kelambakkam, Kanchipuram District, Tamil Nadu, India

    T. Balasubramanian

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  1. S. Shekhar
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  2. A. Sundaramanickam
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  3. K. Saranya
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  4. M. Meena
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  5. S. Kumaresan
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  6. T. Balasubramanian
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Corresponding author

Correspondence to A. Sundaramanickam.

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Editorial responsibility: M. Abbaspour.

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Shekhar, S., Sundaramanickam, A., Saranya, K. et al. Production and characterization of biosurfactant by marine bacterium Pseudomonas stutzeri (SSASM1). Int. J. Environ. Sci. Technol. 16, 4697–4706 (2019). https://doi.org/10.1007/s13762-018-1915-4

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  • DOI: https://doi.org/10.1007/s13762-018-1915-4

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