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Effects of pH, MES, arabinogalactan-proteins on microspore cultures in white cabbage

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Abstract

The objective of this study was to improve induction of embryogenesis in white cabbage (Brassica oleracea var. capitata) microspore cultures. The effect of NLN-13 liquid medium pH on isolated microspore embryogenesis was investigated in five white cabbage genotypes. Relatively high pH (6.2 or 6.4) was more effective on microspore embryogenesis in most of the white cabbage genotypes than the pH of 5.8, especially for inducing microspore-derived embryos in recalcitrant genotype ‘Zhonggan No. 8’. Based on this, 2—(N-Morpholino) ethanesulfonic acid (MES) and the arabinogalactan-protein from gum arabic were tested on four out of five genotypes to see if they could increase embryo yield in microspore cultures. Adding MES or gum arabic alone was effective for these four genotypes, but the frequency of embryos derived from microspores was still low. However, the combination of 10 mg l−1 gum arabic and 3 mM MES in NLN-13 at pH 6.4 significantly enhanced microspore embryogenesis efficiency (with embryo production of 4.57–222.97 embryos per bud), especially with recalcitrant genotype “Zhonggan No. 8” for which it was increased by about 35-fold.

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Abbreviations

MES:

2—(N-Morpholino) ethanesulfonic acid

AGP:

Arabinogalactan-protein

DH:

Doubled haploid

PCD:

Programmed cell death

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Acknowledgments

The above work was funded by Chinese National Natural Science (30871708), Chinese National Key Technology R&D Program (2006BAD13B06), Chinese Ministry of Agriculture ‘948’ Program (2006-G-13A) and Key Laboratory of Horticultural Crop Biology and Germplasm Innovation, Chinese Ministry of Agriculture. We also acknowledge partial funding from the earmarked fund for modern Agro-industry Technology Research System.

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  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Su-xia Yuan, Yan-bin Su, Yu-mei Liu, Zhi-yuan Fang, Li-mei Yang, Mu Zhuang, Yang-yong Zhang & Pei-tian Sun

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  1. Su-xia Yuan
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  2. Yan-bin Su
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  3. Yu-mei Liu
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Correspondence to Yu-mei Liu.

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Yuan, Sx., Su, Yb., Liu, Ym. et al. Effects of pH, MES, arabinogalactan-proteins on microspore cultures in white cabbage. Plant Cell Tiss Organ Cult 110, 69–76 (2012). https://doi.org/10.1007/s11240-012-0131-z

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  • DOI: https://doi.org/10.1007/s11240-012-0131-z

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