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Arabinogalactan proteins are involved in cell aggregation of cell suspension cultures of Beta vulgaris L.

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Abstract

Arabinogalactan proteins (AGPs) are glycoproteins present at cell surfaces. Although exact functions of AGPs remain elusive, they are implicated in plant growth and development. The aim of this study was to evaluate the role of AGPs in the process of cell aggregation of Beta vulgaris L. suspension cultures. It was observed that B. vulgaris suspension cultures accumulated AGPs in parallel form to its cell growth. The AGPs maximum content in the stationary phase was 0.330 mg g−1 dry weight (DW) in the cell wall (CW) and 1.534 mg g−1 DW in the culture medium (CM), generating cell aggregates >500 μm (93.21% DW). The addition of tunicamycin (TM) caused a reduction of AGPs content in CW and CM of 46 and 64%, respectively. These changes were associated with inhibition of growth and the reduction of the cell aggregates >500 μm (50.0% DW). When TM was removed from the CM, cell growth, aggregation, and AGPs content on CW and CM were recovered. Precipitation of AGPs with Yariv reagent generated a reduction of 61.14% of AGPs content in CW and a total inhibition of AGPs secretion in CM. This Yariv treatment generated a reduction in the cell aggregates >500 μm of 51.31% of DW. When the Yariv reagent was removed from the culture, cells did not recover their AGPs accumulation. In addition, cell cultures did not recover their ability to grow and aggregate. These results indicate that AGPs are molecules required in the cellular aggregation process of B. vulgaris L. suspension cultures.

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Abbreviations

AGPs:

Arabinogalactan proteins

CW:

Cell wall

CM:

Culture media

2,4-D:

2,4-Dichlorophenoxyacetic acid

DW:

Dry weight

β-ManY:

β-Manosil Yariv reagent

SPB:

Sodium buffer phosphate

TM:

Tunicamycin

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Acknowledgments

This work was financed by SIP (grants #20100308 and #20100781). J. Capataz-Tafur is indebted to CONACyT for the doctoral fellowship awarded and PIFI-IPN.

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Authors and Affiliations

  1. Departamento de Biotecnología, Centro de Desarrollo de Productos Bióticos del Instituto Politécnico Nacional, Apartado Postal 24, 62731, Yautepec, Morelos, México

    Jacqueline Capataz-Tafur, Gabriela Trejo-Tapia, Mario Rodríguez-Monroy & Gabriela Sepúlveda-Jiménez

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  1. Jacqueline Capataz-Tafur
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  2. Gabriela Trejo-Tapia
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  3. Mario Rodríguez-Monroy
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  4. Gabriela Sepúlveda-Jiménez
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Correspondence to Gabriela Sepúlveda-Jiménez.

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Capataz-Tafur, J., Trejo-Tapia, G., Rodríguez-Monroy, M. et al. Arabinogalactan proteins are involved in cell aggregation of cell suspension cultures of Beta vulgaris L.. Plant Cell Tiss Organ Cult 106, 169–177 (2011). https://doi.org/10.1007/s11240-010-9905-3

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