Abstract
Daucus carota L. cell lines secrete a characteristic set of arabinogalactan proteins (AGPs) into the medium. The composition of this set of AGPs changes with the age of the culture, as can be determined by crossed electrophoresis with the specific AGP-binding agent, β-glucosyl Yariv reagent. Addition of AGPs isolated from the medium of a non-embryogenic cell line to an expiant culture initiated the development of the culture to a non-embryogenic cell line. Without addition of AGPs or with addition of carrot-seed AGPs an embryogenic cell line was established. Three-month-old embryogenic cell lines usually contain less than 30% of dense, highly cytoplasmic cells, i.e. the embryogenic cells, but when carrot-seed AGPs were added this percentage increased to 80%. Addition of carrot-seed AGPs to a two-year-old, non-embryogenic cell line resulted in the re-induction of embryogenic potential. These results show that specific AGPs are essential in somatic embryogenesis and are able to direct development of cells.
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Abbreviations
- AGP:
-
arabinogalactan protein
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
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The authors thank Dr. A.J. Kool (Zaadunie, Enkhuizen, The Netherlands) for scientific and other support and Dr. M.Q.J.M. van Grinsven (Zaadunie, Enkhuizen, The Netherlands) for comments on the manuscript. This research was supported by a grant from the Dutch Ministry of Economic Affairs.
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Kreuger, M., van Holst, GJ. Arabinogalactan proteins are essential in somatic embryogenesis of Daucus carota L.. Planta 189, 243–248 (1993). https://doi.org/10.1007/BF00195083
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DOI: https://doi.org/10.1007/BF00195083