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Analysis of expression profile of selected genes expressed during auxin-induced somatic embryogenesis in leaf base system of wheat (Triticum aestivum) and their possible interactions

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Abstract

Somatic embryogenesis is a notable illustration of plant totipotency and involves reprogramming of development in somatic cells toward the embryogenic pathway. Auxins are key components as their exogenous application recuperates the embryogenic potential of the mitotically quiescent somatic cells. In order to unravel the molecular basis of somatic embryogenesis, cDNA library was made from the regeneration proficient wheat leaf base segments treated with auxin. In total, 1440 clones were sequenced and among these 1,196 good quality sequences were assembled into 270 contigs and 425 were singletons. By reverse northern analysis, a total of 57 clones were found to be upregulated during somatic embryogenesis, 64 during 2,4-D treatment, and 170 were common to 2,4-D treatment and somatic embryogenesis. A substantial number of genes involved in hormone response, signal transduction cascades, defense, anti-oxidation, programmed cell death/senescence and cell division were identified and characterized partially. Analysis of data of select genes suggests that the induction phase of somatic embryogenesis is accompanied by the expression of genes that may also be involved in zygotic embryogenesis. The developmental reprogramming process may in fact involve multiple cellular pathways and unfolding of as yet unknown molecular events. Thus, an interaction network draft using bioinformatics and system biology strategy was constructed. The outcome of a systematic and comprehensive analysis of somatic embryogenesis associated interactome in a monocot leaf base system is presented.

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Acknowledgements

We are grateful to Dr V Ravi for helping with the sequencing of the genes and assembly and thank Ms Rashmi Jain for helping with the in silico analysis. BS acknowledges the award of Senior Research Fellowship from the University Grants Commission (UGC), New Delhi. This research work was financially supported by the Department of Biotechnology, Government of India, and the UGC.

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  1. Interdisciplinary Centre for Plant Genomics & Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India

    Bhumica Singla, Akhilesh K. Tyagi, Jitendra P. Khurana & Paramjit Khurana

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  1. Bhumica Singla
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  2. Akhilesh K. Tyagi
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  3. Jitendra P. Khurana
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Correspondence to Paramjit Khurana.

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11103_2007_9234_MOESM1_ESM.ppt

Magnified version of the rare cold inducible protein, which is the second major regulator in the molecular network (PPT 650 KB)

Magnified version of the cytochrome P450, which is the third major regulator in the molecular network (PPT 802 KB)

The annotated ESTs for the 270 contigs (XLS 49 KB)

The annotated ESTs of 425 singletons (XLS 85 KB)

11103_2007_9234_MOESM5_ESM.xls

Proteins encoded by differentially expressed genes and their expression profile under three experimental conditions shown in Fig. 1, namely, control (A), 2,4-D 24 h (B) and SE (C). Color mark shows expression in a particular stage (XLS 147 KB)

List of the down-regulated ESTs (XLS 21 KB)

11103_2007_9234_MOESM7_ESM.xls

Some representative genes in the putative molecular interaction network draft during somatic embryogenesis in the wheat leaf base system (XLS 202 KB)

Some representative nodes in the putative molecular network draft during SE in wheat leaf base system (XLS 26 KB)

Some representative nodes in the glutathione-S-transferase pathway (XLS 16 KB)

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Singla, B., Tyagi, A.K., Khurana, J.P. et al. Analysis of expression profile of selected genes expressed during auxin-induced somatic embryogenesis in leaf base system of wheat (Triticum aestivum) and their possible interactions. Plant Mol Biol 65, 677–692 (2007). https://doi.org/10.1007/s11103-007-9234-z

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