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Development of eighteen polymorphic microsatellite markers in Scylla paramamosain by 5′ anchored PCR technique

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Abstract

The mud crab Scylla paramamosain plays a significant role in fishery resources in China. In this study, we developed 18 polymorphic microsatellite markers in this important crab by 5′ anchored PCR technique. A total of 125 alleles were detected in a single population of 32 individuals of S. paramamosain. The number of alleles per locus ranged from five to nine, with the allele size ranging from 166 to 316 bp. The polymorphism information content (PIC), observed and expected heterozygosity ranged from 0.39 to 0.88, from 0.33 to 0.92 and from 0.42 to 0.86, respectively. Three loci (Scypa13, Scypa14 and Scypa15) deviated significantly from Hardy–Weinberg equilibrium (HWE) after Bonferroni correction (P < 0.0028), and no linkage disequilibrium was found between loci pairs. These polymorphic microsatellite markers will be useful for the study of population genetic structure, construction of genetic linkage maps and mapping of economically quantitative trait loci (QTL) in S. paramamosain.

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References

  1. Macnae W (1968) A general account of the fauna and flora of mangrove swamps and forests in the Indo-West-Pacific region. Adv Mar Biol 6:73–270

    Article  Google Scholar 

  2. Keenan CP (1999) Aquaculture of the mud crab, genus Scylla-past, present and future. In: Mud Crab Aquaculture and Biology. ACIAR Proceedings, 78: 9–13. Watson Ferguson and Company, Canberra, Australia

  3. Ma LB, Zhang FY, Qiao ZG, He LJ, Ma CY (2006) Sequence analysis of mitochondrial COI gene of Scylla spp. along coast of southeastern China. J Fish China 30(4):463–468 (in Chinese with English abstract)

    CAS  Google Scholar 

  4. Tautz D (1989) Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucleic Acids Res 17(16):6463–6471

    Article  PubMed  CAS  Google Scholar 

  5. Norris AT, Bradley DG, Cunningham EP (2000) Parentage and relatedness determination in farmed Atlantic salmon (Salmo salar) using microsatellite markers. Aquaculture 182:73–83

    Article  Google Scholar 

  6. Divu D, Khushiramani R, Malathi S, Iddya Karunasagar, Indrani Karunasagar (2008) Isolation, characterization and evaluation of microsatellite DNA markers in giant freshwater prawn Macrobrachium rosenbergii, from South India. Aquaculture 284:281–284

    Article  CAS  Google Scholar 

  7. Ju CL, Zhang F, Gao YF, Zhang W, Yan JB, Dai JR, Li JS (2006) Cloning, chromosome mapping and expression analysis of an R2R3-MYB gene under-expressed in maize hybrid. Mol Biol Rep 33:103–110

    Article  PubMed  CAS  Google Scholar 

  8. Zhang LS, Yang CJ, Zhang Y, Li L, Zhang XM, Zhang QL, Xiang JH (2007) A genetic linkage map of Pacific white shrimp (Litopenaeus vannamei): sex-linked microsatellite markers and high recombination rates. Genetica 131:37–49

    Article  PubMed  CAS  Google Scholar 

  9. Jimenez RV, Cruz P, Enriquez RP (2005) Population genetic structure of Pacific White Shrimp (Litopenaeus vannamei) from Mexico to Panama: microsatellite DNA variation. Mar Biotechnol 6:475–484

    Article  Google Scholar 

  10. Cesari M, Luchetti A, Scanabissi F, Mantovani B (2007) Genetic variability in European Leptestheria dahalacensis (Rüppel, 1837) (Crustacea, Branchiopoda, Spinicaudata). Hydrobiologia 586:249–260

    Article  CAS  Google Scholar 

  11. Wu XB, Hu YL (2010) Genetic diversity and molecular differentiation of Chinese toad based on microsatellite markers. Mol Biol Rep 37:2379–2386

    Article  PubMed  CAS  Google Scholar 

  12. Yu HT, Peng YH (2002) Population differentiation and gene flow revealed by microsatellite DNA markers in the house mouse (Mus musculus castaneus) in Taiwan. Zool Sci 19:475–483

    Article  PubMed  CAS  Google Scholar 

  13. Ren JD, Lu LZ, Liu XL, Tao ZR, Zhang CC, Wang DQ, Shen JD, Liu WM, Tian Y, Zhu ZG (2009) Paternity assessment: application on estimation of breeding value in body-weight at first egg trait of egg-laying duck (Anas platyrhynchos). Mol Biol Rep 36:2175–2181

    Article  PubMed  CAS  Google Scholar 

  14. Ikeda N, Bautista NS, Yamada T, Kamijima O, Ishii T (2001) Ultra-simple DNA extraction method for marker-assisted selection using microsatellite markers in rice. Plant Mol Biol Rep 19:27–32

    Article  CAS  Google Scholar 

  15. Sudheer PDVN, Singh S, Mastan SG, Patel J, Reddy MP (2009) Molecular characterization and identification of markers for toxic and non-toxic varieties of Jatropha curcas L. using RAPD, AFLP and SSR markers. Mol Biol Rep 36:1357–1364

    Article  Google Scholar 

  16. Takano M, Barinova A, Sugaya T, Obata Y, Watanabe T, Ikeda M, Taniguchi N (2005) Isolation and characterization of microsatellite DNA markers from mangrove crab, Scylla paramamosain. Mol Ecol Notes 5:794–795

    Article  CAS  Google Scholar 

  17. Xu XJ, Wang GZ, Wang KJ, Li SJ (2009) Isolation and characterization of ten new polymorphic microsatellite loci in the mud crab, Scylla paramamosain. Conserv Genet 10:1877–1878

    Article  CAS  Google Scholar 

  18. Fisher PJ, Gardner RC, Richardson TE (1996) Single locus microsatellites isolated using 5′anchored PCR. Nucleic Acids Res 24(21):4369–4371

    Article  PubMed  CAS  Google Scholar 

  19. Ganley RJ, Bradshaw RE (2001) Rapid identification of polymorphic microsatellite loci in a forest pathogen, Dothistroma pini, using anchored PCR. Mycol Res 105:1075–1078

    Article  CAS  Google Scholar 

  20. Li YQ, Shi TL, Zhang ZW (2007) Development of microsatellite markers from tartary buckwheat. Biotechnol Lett 29:823–827

    Article  PubMed  CAS  Google Scholar 

  21. Tang SJ, Liu ZZ, Tang WQ, Yang JQ (2009) A simple method for isolation of microsatellites from the mudskipper (Boleophthanlmus pectinirostris), without constructing a genomic library. Conserv Genet 10:1957–1959

    Article  CAS  Google Scholar 

  22. Ma HY, Yang JF, Su PZ, Chen SL (2009) Genetic analysis of gynogenetic and common populations of Verasper moseri using SSR markers. Wuhan Univ J Nat Sci 14:267–273

    Article  CAS  Google Scholar 

  23. Li Q, Wan JM (2005) SSRHUNTER: development of a local searching software for SSR sites. Hereditas 27:808–810

    PubMed  Google Scholar 

  24. Bassam BJ, Caetano-Anollés G (1993) Silver staining of DNA in polyacrylamide gels. Appl Biochem Biotechnol 42:181–188

    Article  CAS  Google Scholar 

  25. Yeh FC, Yang RC, Boyle T (1999) POPGENE version 1.31. Microsoft window-based freeware for population genetic analysis. Available: (www.ualberta.ca/~fyeh/). University of Alberta and the Centre for International Forestry Research

  26. Muneer PMA, Gopalakrishnan A, Musammilu KK, Mohindra V, Lal KK, Basheer VS, Lakra WS (2009) Genetic variation and population structure of endemic yellow catfish, Horabagrus brachysoma (Bagridae) among three populations of Western Ghat region using RAPD and microsatellite markers. Mol Biol Rep 36:1779–1791

    Article  PubMed  Google Scholar 

  27. Rice WR (1989) Analyzing tables of statistical tests. Evolution 43(1):223–225

    Article  Google Scholar 

  28. Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 31001106), the science and technology commission of Shanghai municipality (No. 10JC1418600) and the National Non-Profit Institutes (East China Sea Fisheries Research Institute) (No. 2007Z01).

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Authors and Affiliations

  1. Key Lab of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, 200090, China

    Haiyu Cui, Hongyu Ma, Lingbo Ma, Chunyan Ma & Qunqun Ma

  2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China

    Haiyu Cui & Qunqun Ma

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  1. Haiyu Cui
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  2. Hongyu Ma
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  3. Lingbo Ma
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  4. Chunyan Ma
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  5. Qunqun Ma
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Correspondence to Lingbo Ma.

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Cui, H., Ma, H., Ma, L. et al. Development of eighteen polymorphic microsatellite markers in Scylla paramamosain by 5′ anchored PCR technique. Mol Biol Rep 38, 4999–5002 (2011). https://doi.org/10.1007/s11033-010-0645-6

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  • DOI: https://doi.org/10.1007/s11033-010-0645-6

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