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Genetic analysis of gynogenetic and common populations of Verasper moseri using SSR markers

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Wuhan University Journal of Natural Sciences

Abstract

The genetic structure and variation of the artificial meio- gynogenetic population and common population of barfin flounder (Verasper moseri) were analyzed using eight microsatellite markers. A total of 29 alleles were detected, of which 23 alleles were in the artificial gynogenetic population while 29 alleles were in the control group. The average observed heterozygosity (H O) of eight loci in the control group (0.526 8) was several times higher than that (0.185 8) in the gynogenetic population. The results indicate that the genetic diversity of the control group was much higher than that of the gynogenetic population of barfin flounder (Verasper moseri). Most loci significantly deviated from Hardy-Weinberg equilibrium (HWE) after Bonferroni correction (p < 0.005 56) in the gynogenetic population, while four loci deviated from HWE in the control group. The coefficient of gene differentiation (G ST) was 0.131 0, and the genetic distance was 0.171 8 between the two populations, suggesting a significant genetic differentiation between the two populations.

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Authors and Affiliations

  1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Qingdao, 266071, Shandong, China

    Hongyu Ma, Jingfeng Yang, Pengzhi Su & Songlin Chen

  2. College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, Shandong, China

    Hongyu Ma

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  1. Hongyu Ma
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  2. Jingfeng Yang
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  3. Pengzhi Su
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  4. Songlin Chen
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Correspondence to Songlin Chen.

Additional information

Foundation item: Supported by the National High Technology Research and Development Program of China (863 Program) (2006AA10A403), Shandong Genetic Improvement Key Project for Agricultural Organism, and Taishan Scholar Project of Shandong Province

Biography: MA Hongyu (1979–), male, Ph. D. candidate, research direction: marine biotechnology and molecular genetics.

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Ma, H., Yang, J., Su, P. et al. Genetic analysis of gynogenetic and common populations of Verasper moseri using SSR markers. Wuhan Univ. J. Nat. Sci. 14, 267–273 (2009). https://doi.org/10.1007/s11859-009-0315-5

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  • DOI: https://doi.org/10.1007/s11859-009-0315-5

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