Abstract
We prevent an ultra-simple DNA extraction method for microsatellite analysis of rice. Each extraction requires only one microtube, one disposable pipette tip, TE buffer and few pieces (about 5 mm) of rice leaf tissue. This is sufficient for 200 PCR reactions. The extract can be kept in the freezer for long-term storage. Also, DNA can be extracted from 200–300 individuals in a few hours. These features enabled us to perform rapid largescale seedling genotyping required for marker-assisted selection. We have also examined the applicability of this method for other PCR-based markers: RAPDs, nuclear STS, chloroplast STS and chloroplast microsatellites.
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Abbreviations
- QTL:
-
quantitative trait locus
- RAPD:
-
random amplified polymorphic DNA
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Ikeda, N., Bautista, N.S., Yamada, T. et al. Ultra-simple DNA extraction method for marker-assisted selection using microsatellite markers in rice. Plant Mol Biol Rep 19, 27–32 (2001). https://doi.org/10.1007/BF02824075
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DOI: https://doi.org/10.1007/BF02824075