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The use of species-specific DNA markers for assessing alien chromosome transfer in Brassica rapa and Brassica oleracea-monosomic additions of Raphanus sativus

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Abstract

Monosomic addition lines (MALs) are useful materials not only for cytogenetic and molecular genetic studies but also for plant breeding as gene sources. In our previous study, two MALs in the tribe Brassiceae were developed, one being Raphanus sativus lines with alien chromosomes of Brassica rapa (B. rapa-monosomic addition lines; BrMALs) and the second being those with alien chromosomes of Brassica oleracea (B. oleracea-monosomic addition lines; BoMALs). We developed species-specific DNA markers from the genomic sequences of B. rapa and B. oleracea comparing them with those of R. sativus, and identified chromosomes added in BrMALs and BoMALs using these markers. It was revealed that eight types of BrMALs have seven chromosomes of B. rapa and seven types of BoMALs have six chromosomes of B. oleracea. Furthermore, chromosome breakage and homoeologous recombination were suggested to have occurred in some MALs. The developed species-specific DNA markers are considered to be useful for producing MALs and also for assessing chromosome abnormality in MALs.

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Acknowledgments

This work was supported by the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN). KT is a recipient of the JSPS fellowship for young scientists (DC).

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Authors and Affiliations

  1. Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, Miyagi, 981-8555, Japan

    Kaoru Tonosaki, Hiroyasu Kitashiba & Takeshi Nishio

  2. Faculty of Agriculture, Utsunomiya University, 350 Minemachi, Utsunomiya, 321-8505, Japan

    Michiko Akaba, Sang Woo Bang & Yukio Kaneko

Authors
  1. Kaoru Tonosaki
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  2. Michiko Akaba
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  3. Sang Woo Bang
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  4. Hiroyasu Kitashiba
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  5. Yukio Kaneko
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  6. Takeshi Nishio
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Correspondence to Takeshi Nishio.

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Tonosaki, K., Akaba, M., Bang, S.W. et al. The use of species-specific DNA markers for assessing alien chromosome transfer in Brassica rapa and Brassica oleracea-monosomic additions of Raphanus sativus . Mol Breeding 34, 1301–1311 (2014). https://doi.org/10.1007/s11032-014-0117-0

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  • DOI: https://doi.org/10.1007/s11032-014-0117-0

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