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Generation and characterization of Brassica rapa ssp. pekinensis – B. oleracea var. capitata monosomic and disomic alien addition lines

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Abstract

Five monosomic alien addition lines (MAALs) of Brassica rapa ssp. pekinensis – B. oleracea var. capitata were obtained by hybridization and backcrossing between B. rapa ssp. pekinensis (female parent) and B. oleracea var. capitata. The alien linkage groups were identified using 42 B. oleracea var. capitata linkage group-specific markers as B. oleracea linkage groups C2, C3, C6, C7 and C8. Based on the chromosomal karyotype of root tip cells, these five MAALs added individual chromosomes from B. oleracea var. capitata: chr 1 (the longest), chr 2 or 3, chr 5 (small locus of 25S rDNA), chr 7 (satellite-carrying) and chr 9 (the shortest). Five disomic alien addition lines were then generated by selfing their corresponding MAALs.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (31101552, 30871713), the Research Fund for the Doctoral Programme of Higher Education in China (20101302120006) and the Natural Science Foundation of Hebei Province (C2014204093). The authors are grateful to Priscilla Licht for critical reading of the manuscript.

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Authors and Affiliations

  1. College of Horticulture, Agricultural University of Hebei, No. 289, Lingyusi Road, Baoding, 071001, People’s Republic of China

    AI XIA GU, SHU XING SHEN, YAN HUA WANG, JIAN JUN ZHAO, SHU XIN XUAN, XUE PING CHEN, XIAO FENG LI, SHUANG XIA LUO & YU JING ZHAO

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  1. AI XIA GU
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  2. SHU XING SHEN
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  3. YAN HUA WANG
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Correspondence to AI XIA GU.

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[Gu A. X., Shen S. X.,Wang Y. H., Zhao J. J., Xuan S. X., Chen X. P., Li X. F., Luo S. X. and Zhao Y. J. 2015 Generation and characterization of Brassica rapa ssp. pekinensis – B. oleracea var. capitata monosomic and disomic alien addition lines. J. Genet. 94, xx–xx]

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GU, A.X., SHEN, S.X., WANG, Y.H. et al. Generation and characterization of Brassica rapa ssp. pekinensis – B. oleracea var. capitata monosomic and disomic alien addition lines. J Genet 94, 435–444 (2015). https://doi.org/10.1007/s12041-015-0542-9

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  • DOI: https://doi.org/10.1007/s12041-015-0542-9

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