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Identification of molecular markers linked to trilocular gene (mc1) in Brassica juncea L.

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Abstract

Multilocular plants generally have higher yield than bilocular plants, which is attributable to the difference in the number of seeds per silique. In this study, we investigated a landrace of Brassica juncea in China that has trilocular siliques. We discovered that two independent recessive genes (mc1 and mc2) controlled the trait of trilocular silique. Mc1 was preliminarily mapped using amplified fragment length polymorphism (AFLP) technology in combination with bulked segregant analysis. From a survey of 2,048 AFLP primer combinations, we obtained 24 AFLP markers linked to the target gene, of which five were successfully converted into sequence-characterized amplified region markers. Two of these five AFLP markers and two other AFLP markers shared high sequence homology with A07 in the Brassica rapa genome. We developed seven simple sequence repeat primer pairs based on their sequence homology with A07. Our result also demonstrated that EC14MC14 and SC20 are the closest markers flanking the Mc1 gene, at distances of 1.1 and 1.6 cM, respectively. These molecular markers will facilitate the fine mapping and cloning of the mc1 gene and accelerate the selection process for multilocular rapeseed through marker-assisted selection.

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Acknowledgments

This research was supported by funds from the National High-Tech R&D Program (863 Program) (grant number 2011AA10A104) and the Program for Modern Agricultural Industrial Technology System (nycytx-00501). The authors are grateful to Dr. Youlian Pan from the Institute for Information Technology, National Research Council of Canada and Dr. Yanxiu Liu from the English Department of Huazhong Agricultural University in China for critically reading the manuscript.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Huazhong Agricultural University, Wuhan, 430070, China

    Ping Xu, Zewen Lv, Xiangxiang Zhang, Xiaohua Wang, Yuanyuan Pu, Hongmei Wang, Bin Yi, Jing Wen, Chaozhi Ma, Jinxing Tu, Tingdong Fu & Jinxiong Shen

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  1. Ping Xu
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  2. Zewen Lv
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  3. Xiangxiang Zhang
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  4. Xiaohua Wang
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  5. Yuanyuan Pu
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  6. Hongmei Wang
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  7. Bin Yi
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  9. Chaozhi Ma
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  10. Jinxing Tu
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  11. Tingdong Fu
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  12. Jinxiong Shen
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Correspondence to Jinxiong Shen.

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Xu, P., Lv, Z., Zhang, X. et al. Identification of molecular markers linked to trilocular gene (mc1) in Brassica juncea L.. Mol Breeding 33, 425–434 (2014). https://doi.org/10.1007/s11032-013-9960-7

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  • DOI: https://doi.org/10.1007/s11032-013-9960-7

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