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Genetic linkage maps of white birches (Betula platyphylla Suk. and B. pendula Roth) based on RAPD and AFLP markers

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Abstract

A pseudo-testcross mapping strategy was used in combination with the random amplified polymorphism DNA (RAPD) and amplified fragment length polymorphism (AFLP) genotyping methods to develop two moderately dense genetic linkage maps for Betula platyphylla Suk. (Asian white birch) and B. pendula Roth (European white birch). Eighty F1 progenies were screened with 291 RAPD markers and 451 AFLP markers. We selected 230 RAPD and 362 AFLP markers with 1:1 segregation and used them for constructing the parent-specific linkage maps. The resultant map for B. platyphylla was composed of 226 markers in 24 linkage groups (LGs), and spanned 2864.5 cM with an average of 14.3 cM between adjacent markers. The linkage map for B. pendula was composed of 226 markers in 23 LGs, covering 2489.7 cM. The average map distance between adjacent markers was 13.1 cM. Clustering of AFLP markers was observed on several LGs. The availability of these white birch linkage maps will contribute to the molecular genetics and the implementation of marker-assisted selection in these important forest species.

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Authors and Affiliations

  1. Key Laboratory of Forest Tree Genetic Improvement and Biotechnology of Ministry of Education, Northeast Forestry University, 26 Hexing Road, Dongli District, Harbin, 150040, People’s Republic of China

    Tingbo Jiang, Boru Zhou & Fuling Gao

  2. Crop Protection and Management Research Unit, USDA-Agricultural Research Service, University of Georgia, Tifton, GA, 31793, USA

    Baozhu Guo

Authors
  1. Tingbo Jiang
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  2. Boru Zhou
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  3. Fuling Gao
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  4. Baozhu Guo
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Correspondence to Tingbo Jiang.

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Jiang, T., Zhou, B., Gao, F. et al. Genetic linkage maps of white birches (Betula platyphylla Suk. and B. pendula Roth) based on RAPD and AFLP markers. Mol Breeding 27, 347–356 (2011). https://doi.org/10.1007/s11032-010-9436-y

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