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Genetic characterization of powdery mildew resistance in U.S. hard winter wheat

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Abstract

Powdery mildew significantly affects grain yield and end-use quality of winter wheat in the southern Great Plains. Employing resistance resources in locally adapted cultivars is the most effective means to control powdery mildew. Two types of powdery mildew resistance exist in wheat cultivars, i.e., qualitative and quantitative. Qualitative resistance is controlled by major genes, is race-specific, is not durable, and is effective in seedlings and in adult plants. Quantitative resistance is controlled by minor genes, is non-race-specific, is durable, and is predominantly effective in adult plants. In this study, we found that the segregation of powdery mildew resistance in a population of recombinant inbred lines developed from a cross between the susceptible cultivar Jagger and the resistant cultivar 2174 was controlled by a major QTL on the short arm of chromosome 1A and modified by four minor QTLs on chromosomes 1B, 3B, 4A, and 6D. The major QTL was mapped to the genomic region where the Pm3 gene resides. Using specific PCR markers for seven Pm3 alleles, 2174 was found to carry the Pm3a allele. Pm3a explained 61% of the total phenotypic variation in disease reaction observed among seedlings inoculated in the greenhouse and adult plants grown in the field and subjected to natural disease pressure. The resistant Pm3a allele was present among 4 of 31 cultivars currently being produced in the southern Great Plains. The genetic effects of several minor loci varied with different developmental stages and environments. Molecular markers associated with these genetic loci would facilitate incorporating genetic resistance to powdery mildew into improved winter wheat cultivars.

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Acknowledgments

This study was supported by the National Research Initiative of the USDA-Cooperative State Research, Education, and Extension Service (CAP grant 2006-55606-16629), by the Oklahoma Wheat Research Foundation, and by the Oklahoma Center of Advanced Science and Technology (OCAST). This research project was partially funded by the Oklahoma Agricultural Experiment Station.

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Authors and Affiliations

  1. Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK, 74078, USA

    Yihua Chen, Brett F. Carver, Hailin Zhang & Liuling Yan

  2. Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA

    Robert M. Hunger

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  1. Yihua Chen
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  2. Robert M. Hunger
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  3. Brett F. Carver
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  4. Hailin Zhang
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  5. Liuling Yan
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Correspondence to Liuling Yan.

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Chen, Y., Hunger, R.M., Carver, B.F. et al. Genetic characterization of powdery mildew resistance in U.S. hard winter wheat. Mol Breeding 24, 141–152 (2009). https://doi.org/10.1007/s11032-009-9279-6

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  • DOI: https://doi.org/10.1007/s11032-009-9279-6

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