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Genetic mapping and localization of a major QTL for seedling resistance to downy mildew in Chinese cabbage (Brassica rapa ssp. pekinensis)

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Abstract

Downy mildew caused by the fungus Peronospora parisitica is a serious threat to members of the Brassicaceae family. Annually, a substantial loss of yield is caused by the widespread presence of this disease in warm and humid climates. The aim of this study was to localize the genetic factors affecting downy mildew resistance in Chinese cabbage (Brassica rapa ssp. pekinensis). To achieve this goal, we improved a preexisting genetic map of a doubled-haploid population derived from a cross between two diverse Chinese cabbage lines, 91-112 and T12-19, via microspore culture. Microsatellite simple sequence repeat (SSR) markers, isozyme markers, sequence-related amplified polymorphism markers, sequence-characterized amplified region markers and sequence-tagged-site markers were integrated into the previously published map to construct a composite Chinese cabbage map. In this way, the identities of linkage groups corresponding to the Brassica A genome reference map were established. The new map contains 519 markers and covers a total length of 1,070 cM, with an average distance between markers of 2.06 cM. All markers were designated as A1–A10 through alignment and orientation using 55 markers anchored to previously published B. rapa or B. napus reference maps. Of the 89 SSR markers mapped, 15 were newly developed from express sequence tags in Genbank. The phenotypic assay indicated that a single major gene controls seedling resistance to downy mildew, and that a major QTL was detected on linkage group A8 by both interval and MQM mapping methods. The RAPD marker K14-1030 and isozyme marker PGM flanked this major QTL in a region spanning 2.9 cM, and the SSR marker Ol12G04 was linked to this QTL by a distance of 4.36 cM. This study identified a potential chromosomal segment and tightly linked markers for use in marker-assisted selection to improve downy mildew resistance in Chinese cabbage.

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Acknowledgments

The authors wish to thank two anonymous reviewers for their constructive comments regarding the manuscript. This work was supported in part by grants from the National High Technology Research and Development Program of China (863 program) (No. 2006AA10Z1C9), the Beijing Natural Science Foundation, China (No. 5062007 and 5083020), the Beijing Nova Programme, China (No. 2006B05) and the National Natural Science Foundation of China (No. 30671422).

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  1. Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing Vegetable Research Center (BVRC), Beijing, 100097, China

    Shuancang Yu, Fenglan Zhang, Renbo Yu, Yanmin Zou, Jiani Qi, Xiuyun Zhao, Yangjun Yu, Deshuang Zhang & Li Li

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  1. Shuancang Yu
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  2. Fenglan Zhang
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  3. Renbo Yu
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Correspondence to Fenglan Zhang.

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Yu, S., Zhang, F., Yu, R. et al. Genetic mapping and localization of a major QTL for seedling resistance to downy mildew in Chinese cabbage (Brassica rapa ssp. pekinensis). Mol Breeding 23, 573–590 (2009). https://doi.org/10.1007/s11032-009-9257-z

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