Abstract
The use of clubroot resistance (CR) genes is an effective and economical approach for controlling Plasmodiophora brassicae, the causal agent of clubroot disease in Chinese cabbage (Brassica rapa) and other Brassica crops. In a previous study, we identified and mapped the CRb locus on chromosome A03 of B. rapa in the doubled-haploid (DH) line ‘CR Shinki DH line’ of Chinese cabbage. In this study, CRb, a dominant gene conferring resistance to pathotype 4 of P. brassicae, was finely mapped in combination with bulked segregant analysis and bioinformatics analysis (BIA). Using 1,486 highly susceptible individuals and 2,896 individuals from two separate F2 populations of ‘702-5’ (B. rapa ssp. chinensis) × ‘CR Shinki DH line,’ the CRb locus was narrowed to a region of approximately 0.14 cM between two flanking markers, TCR79 and TCR108. The sequences of seven newly developed markers linked to CRb were landed on bacterial artificial chromosome (BAC) of the reference B. rapa ‘Chiifu-401-42’ by BIA, and a physical map consisting of three BAC clones was constructed. The CRb locus was defined as an interval of approximately 83.5 kb on a BAC clone (KBrB085J21). The target interval contained one Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR–NBS–LRR) gene, one NBS–LRR gene, and several putative regulatory genes in the B. rapa genome. The CRb gene was tightly linked to two other CR genes, CRa and CRb Kato. These results provide useful information for isolation of the CRb gene and tightly linked molecular markers for breeding CR in B. rapa.
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Acknowledgments
This study was supported by grants from the National Natural Scientific Foundation of China, Project No. 31171967, the Natural Science Foundation of Liaoning Province of China, Project No. 20102199, the Research Fund for the Doctoral Program of Higher Education of China, Project No. 20132103110010, and Golden Seed Project sponsored by Ministry of Agriculture, Food and Rural Affairs, Ministry of Oceans and Fisheries, Rural Development Administration and Korea Forest Service of Korea, Project No. 213002-04.
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The authors declare that they have no conflict of interest.
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The experiments comply with the current laws of China.
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Teng Zhang and Zuo Zhao have contributed equally to this work.
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Zhang, T., Zhao, Z., Zhang, C. et al. Fine genetic and physical mapping of the CRb gene conferring resistance to clubroot disease in Brassica rapa . Mol Breeding 34, 1173–1183 (2014). https://doi.org/10.1007/s11032-014-0108-1
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DOI: https://doi.org/10.1007/s11032-014-0108-1