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Isolation of protoplasts from Fucus serratus and F. vesiculosus(Fucales, Phaeophyceae): factors affecting protoplast yield

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Abstract

Protoplasts were isolated enzymatically from meristematic tissues of the brown algae, Fucus serratus, using a combination of 2% cellulase R-10 Onozuka, 0.5% macerozyme and 1% crude extract of gland gut of Aplysia vaccaria. The main factors affecting protoplast yield were identified. Protoplasts were produced in large quantities from apical region of thallus and from plantlets compared to mature explants. Yields were greatly improved by the addition of sodium citrate and bovine serum albumin in the enzymatic solution and could reach 5.8 × 106 protoplasts per gram of fresh wt. The applicability of these optimal parameters to other species Fucus vesiculosus was shown.

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  1. Laboratoire de Biologie et de Biotechnologies Marines, UMR IFREMER 100 Physiologie et Ecophysiologie des Mollusques Marins, Université de Caen Basse-Normandie, BP 5136, Esplanade de la Paix, 14032, Caen cedex 5, France

    Isabelle Mussio & Anne-Marie Rusig

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  1. Isabelle Mussio
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  2. Anne-Marie Rusig
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Correspondence to Isabelle Mussio.

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Mussio, I., Rusig, AM. Isolation of protoplasts from Fucus serratus and F. vesiculosus(Fucales, Phaeophyceae): factors affecting protoplast yield. J Appl Phycol 18, 733–740 (2006). https://doi.org/10.1007/s10811-006-9079-x

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  • DOI: https://doi.org/10.1007/s10811-006-9079-x

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