Abstract;
Buccal juice of the sea hare Aplysia juliana was found to degrade algal polysaccharides. The optimal enzyme composition for protoplast preparation from Undaria pinnatifida was protein at 48 μg/ml buccal juice from sea hare, 10 mg/ml cellulase Onozuka-RS, 0.4 M NaCl, 0.8 M sorbitol, 2 mg/ml dextran sulfate sodium salt, and 1 μl/ml 2-mercaptoethanol in 10 mM MES buffer (pH 6.0). Protoplasts of Eisenia bicyclis, Endarachne binghamiae (Phaeophyta), and Ulva pertusa (Chlorophyta) could also be prepared in a similar manner. Yields of these protoplasts were about 107 cells per gram of fresh weight alga.
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Received January 26, 1998; accepted September 17, 1998.
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Wakabayashi, T., Kuboi, T., Tuboi, T. et al. Preparation of High Yields of Algal Protoplasts Using Buccal Juice of Sea Hare and Commercial Cellulase. Mar. Biotechnol. 1, 407–410 (1999). https://doi.org/10.1007/PL00011796
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DOI: https://doi.org/10.1007/PL00011796