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Protoplast production in Chondrus crispus gametophytes (gigartinales, rhodophyta)

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Abstract

Protoplasts were isolated from female gametophytes of Chondrus crispus (Stackh.) using commercial cellulase and various carrageenases prepared from marine bacteria. Depending on the nature of the donor tissue (apices or whole thallus, wild or cultivated strains), yields ranged from 1.0–8.5×108 protoplasts per gram of fresh tissue. Preincubating the tissue with a potassium chelator, Kryptofix 222, enhanced protoplast yields by 30–50 %. Based on staining with fluorescein diacetate most protoplasts were viable. A few protoplasts regenerated a cell wall and divided.

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Authors and Affiliations

  1. Centre d'Etudes Océanologiques et de Biologie Marine, CNRS UPR 4601, F-29211, Roscoff, France

    Y. Le Gall & B. Kloareg

  2. Sanofi Bio-Industries, Usine de Baupte, F-50500, Carentan, France

    J. P. Braud

Authors
  1. Y. Le Gall
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  2. J. P. Braud
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  3. B. Kloareg
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Communicated by A. M. Boudet

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Le Gall, Y., Braud, J.P. & Kloareg, B. Protoplast production in Chondrus crispus gametophytes (gigartinales, rhodophyta). Plant Cell Reports 8, 582–585 (1990). https://doi.org/10.1007/BF00270058

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  • DOI: https://doi.org/10.1007/BF00270058

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