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Molecular and functional characterization of the JcMYB1, encoding a putative R2R3-MYB transcription factor in Jatropha curcas

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Abstract

The cDNA encoding the R2R3MYB transcription factor, designated as JcMYB1, was isolated from Jatropha curcas using rapid amplification of cDNA ends. JcMYB1 contains a 942 bp open reading frame that encodes 313 amino acids. The deduced JcMYB1 protein was predicted to possess the conserved R2R3 domain and the signature motif specific for the interaction between MYB and bHLH proteins in the R3 domain. JcMYB1 is a member of the R2R3-MYB transcription factor subfamily. JcMYB1 expressed at different levels with the highest transcription in the roots, followed by the leaves and stems. JcMYB1 transcription was up-regulated by drought, polyethylene glycol, NaCl and cold treatments. JcMYB1 transcription was up-regulated by abscisic acid and jasmonic acid treatment, whereas JcMYB1 expression was not induced by ethylene. JcMYB1 over-expression improved the drought and salt stress tolerance of transgenic tobacco. JcMYB1 has an important function in modulating responses to abiotic stresses.

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Abbreviations

ABA:

Abscisic acid

bHLH:

Basic helix-loop-helix

GFP:

Green fluorescent protein

JA:

Jasmonic acid

ORF:

Open reading frame

PEG:

Polyethylene glycol

RACE:

Rapid amplification of cDNA ends

RT-PCR:

Reverse transcription polymerase chain reaction

TF:

Transcription factor

UTR:

Untranslated region

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Acknowledgments

This work was financially supported by the National Basic Research and Development Program (2010CB126603) and the Major Technology Project of Hainan (ZDZX2013023-1).

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Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

    Hui-Liang Li, Dong Guo & Shi-Qing Peng

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  1. Hui-Liang Li
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  2. Dong Guo
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Correspondence to Shi-Qing Peng.

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Li, HL., Guo, D. & Peng, SQ. Molecular and functional characterization of the JcMYB1, encoding a putative R2R3-MYB transcription factor in Jatropha curcas . Plant Growth Regul 75, 45–53 (2015). https://doi.org/10.1007/s10725-014-9930-z

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