Abstract
The Arabidopsis thaliana transcription factor gene AtMYB44 was induced within 10 min by treatment with methyl jasmonate (MeJA). Wound-induced expression of the gene was observed in local leaves, but not in distal leaves, illustrating jasmonate-independent induction at wound sites. AtMYB44 expression was not abolished in Arabidopsis mutants insensitive to jasmonate (coi1), ethylene (etr1), or abscisic acid (abi3-1) when treated with the corresponding hormones. Moreover, various growth hormones and sugars also induced rapid AtMYB44 transcript accumulation. Thus, AtMYB44 gene activation appears to not be induced by any specific hormone. MeJA-induced activation of jasmonate-responsive genes such as JR2, VSP, LOXII, and AOS was attenuated in transgenic Arabidopsis plants overexpressing the gene (35S:AtMYB44), but significantly enhanced in atmyb44 knockout mutants. The 35S:MYB44 and atmyb44 plants did not show defectiveness in MeJA-induced primary root growth inhibition, indicating that the differences in jasmonate-responsive gene expression observed was not due to alterations in the jasmonate signaling pathway. 35S:AtMYB44 seedlings exhibited slightly elevated chlorophyll levels and less jasmonate- induced anthocyanin accumulation, demonstrating suppression of jasmonate-mediated responses and enhancement of ABA-mediated responses. These observations support the hypothesis of mutual antagonistic actions between jasmonate- and abscisic acid-mediated signaling pathways.
Similar content being viewed by others
References
Anderson, J.P., Badruzsaufari, E., Schenk, P.M., Manners, J.M., Desmond, O.J., Ehlert, C., Maclean, D.J., Ebert, P.R., and Kazan, K. (2004). Antagonistic interaction between abscisic acid and jasmonate-ethylene signaling pathways modulates defense gene expression and disease resistance in Arabidopsis. Plant Cell 16, 3460–3479.
Berrocal-Lobo, M., Molina, A., and Solano, R. (2002). Constitutive expression of ETHYLENE-RESPONSIVE-FACTOR1 in Arabidopsis confers resistance to several necrotrophic fungi. Plant J. 29, 23–32.
Carpenter, C.D., and Simon, A.E. (1998). Preparation of RNA. Methods Mol. Biol. 82, 85–89.
Cheong, J.-J., and Choi, Y.D. (2003). Methyl jasmonate as a vital substance in plants. Trends Genet. 19, 409–413.
Cheong, J.-J., and Choi, Y.D. (2007). Signaling pathways for the biosynthesis and action of jasmonates. J. Plant Biol. 50, 122–131.
Chini, A., Fonseca, S., Fernández, G., Adie, B., Chico, J.M., Lorenzo, O., García-Casado, G., López-Vidriero, I., Lozano, F.M., Ponce, M.R., et al. (2007). The JAZ family of repressors is the missing link in jasmonate signaling. Nature 448, 666–671.
Creelman, R.A., and Rao, M.V. (2002). The oxylipin pathway in Arabidopsis. In: Somerville, C.R., and Meyerowitz, E.M. eds., The Arabidopsis book, American Society of Plant Biologists. DOI 10.1199/tab.0012. (http://www.aspb.org/publications/Arabidopsis/).
Farmer, E.E., Alméras, E., and Krishnamurthy, V. (2003). Jasmonates and related oxylipins in plant responses to pathogenesis and herbivory. Curr. Opin. Plant Biol. 6, 372–378.
Finkelstein, R.R., Gampala, S.S.L., and Rock, C.D. (2002). Abscisic acid signaling in seeds and seedlings. Plant Cell 14, S15–S45.
Fujita, M., Fujita, Y., Noutoshi, Y., Takahashi, F., Narusaka, Y., Yamaguchi-Shinozaki, K., and Shinozaki, K. (2006). Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks. Curr. Opin. Plant Biol. 9, 436–442.
Grant, M., and Lamb, C. (2006). Systemic immunity. Curr. Opin. Plant Biol. 9, 414–420.
Holton, T.A., and Cornish, E.C. (1995). Genetics and biochemistry of anthocyanin biosynthesis. Plant Cell 7, 1071–1083.
Hung, K.T., Cheng, D.G., Hsu, Y.T., and Kao, C.H. (2007). Abscisic acid-induced hydrogen peroxide is required for anthocyanin accumulation in leaves of rice seedlings. J. Plant Physiol. 165, 1280–1287.
Jung, S. (2004). Effect of chlorophyll reduction in Arabidopsis thaliana by methyl jasmonate or norflurazon on antioxidant systems. Plant Physiol. Biochem. 42, 225–231.
Jung, C., Lyou, S.H., Yeu, S.Y., Kim, M.A., Rhee, S., Kim, M., Lee, J.S., Choi, Y.D., and Cheong, J.-J. (2007a). Microarray-based screening of jasmonate-responsive genes in Arabidopsis thaliana. Plant Cell Rep. 26, 1053–1063.
Jung, C., Yeu, S.Y., Koo, Y.J., Kim, M., Choi, Y.D., and Cheong, J.-J. (2007b). Transcript profile of transgenic Arabidopsis constitutively producing methyl jasmonate. J. Plant Biol. 50, 12–17.
Jung, C., Seo, J.S., Han, S.W., Koo, Y.J., Kim, C.H., Song, S.I., Nahm, B.H., Choi, Y.D., and Cheong, J.-J. (2008). Overexpression of AtMYB44 enhances stomata closure to confer abiotic stress tolerance in transgenic Arabidopsis. Plant Physiol. 146, 623–635.
Kariola, T., Brader, G., Li, J., and Palva, E.T. (2005). Chlorophyllase 1, a damage control enzyme, affects the balance between defense pathways in plants. Plant Cell 17, 282–294.
Kim, J., Yi, H., Choi, G., Shin, B., Song, P.-J., and Choi, G. (2003). Functional characterization of phytochrome interacting factor 3 in phytochrome-mediated light signal transduction. Plant Cell 15, 2399–2407.
Kranz, H.D., Denekamp, M., Greco, R., Jin, H., Leyva, A., Meissner, R.C., Petroni, K., Urzainqui, A., Bevan, M., Martin, C., et al. (1998). Towards functional characterization of the members of the R2R3-MYB gene family from Arabidopsis thaliana. Plant J. 16, 263–276.
Kunkel, B.N., and Brooks, D.B. (2002). Cross talk between signaling pathways in pathogen defense. Curr. Opin. Plant Biol. 5, 325–331.
León, J., Rojo, E., Titarenko, E., and Sánchez-Serrano, J.J. (1998). Jasmonic acid-dependent and -independent wound signal transduction pathways are differentially regulated by Ca2+/calmodulin in Arabidopsis thaliana. Mol. Gen. Genet. 258, 412–419.
Lichtenthaler, H.K. (1987). Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Method Enzymol. 148, 350–382.
Lorenzo, O., Piqueras, R., Sánchez-Serrano, J.J., and Solano, R. (2003). ETHYLENE RESPONSE FACTOR1 integrates signals from ethylene and jasmonate pathways in plant defense. Plant Cell 15, 165–178.
Lorenzo, O., Chico, J.M., Sánchez-Serrano, J.J., and Solano, R. (2004). JASMONATE-INSENSITIVE1 encodes a MYC transcription factor essential to discriminate between different jasmonate-regulated defense responses in Arabidopsis. Plant Cell 16, 1938–1950.
Lorenzo, O., and Solano, R. (2005). Molecular players regulating the jasmonate signaling network. Curr. Opin. Plant Biol. 8, 1–9.
Mancinelli, A.L. (1990). Interaction between light quality and light quantity in the photoregulation of anthocyanin production. Plant Physiol. 92, 1191–1195.
Mason, H.S., and Mullet, J.E. (1990). Expression of two soybean vegetative storage protein genes during development and in response to water deficit, wounding, and jasmonic acid. Plant Cell 2, 569–579.
Moons, A., Prinsen, E., Bauw, G., and Van Montagu, M. (1997). Antagonistic effects of abscisic acid and jasmonates on saltinducible transcripts in rice roots. Plant Cell 9, 2243–2259.
Penninckx, I.A.M.A., Thomma, B.P.H.J., Buchala, A., Métraux, J.-P., and Broekaert, W.F. (1998). Concomitant activation of jasmonate and ethylene response pathways is required for induction of a plant defensin gene in Arabidopsis. Plant Cell 10, 2103–2113.
Rojo, E., León, J., and Sánchez-Serrano, J.J. (1999). Cross-talk between wound signaling pathways determines local versus systemic gene expression in Arabidopsis thaliana. Plant J. 20, 135–142.
Staswick, P.E., Su, W., and Howell, S.H. (1992). Methyl jasmonate inhibition of root growth and induction of a leaf protein are decreased in an Arabidopsis thaliana mutant. Proc. Natl. Acad. Sci. USA. 89, 6837–6840.
Staswick, P.E., and Tiryaki, I. (2004). The oxylipin signal jasmonic acid is activated by an enzyme that conjugates it to isoleucine in Arabidopsis. Plant Cell 16, 2117–2127.
Thines, B., Katsir, L., Melotto, M., Niu, Y., Mandaokar, A., Liu, G., Nomura, K., He, S.Y., Howe, G.A., and Browse, J. (2007). JAZ repressor proteins are targets of the SCFCOI1 complex during jasmonate signaling. Nature 448, 661–665.
Titarenko, E., Rojo, E., León, J., and Sánchez-Serrano, J.J. (1997). Jasmonic acid-dependent and -independent signaling pathways control wound-induced gene activation in Arabidopsis thaliana. Plant Physiol. 115, 817–826
Tsuchiya, T., Ohta, H., Okawa, K., Iwamatsu, A., Shimada, H., Masuda, T., and Takamiya, K.-I. (1999). Cloning of chlorophyllase, the key enzyme in chlorophyll degradation: finding of a lipase motif and the induction by methyl jasmonate. Proc. Natl. Acad. Sci. USA 96, 15362–15367.
Wang, K.L.-C., Li, H., and Ecker, J.R. (2002). Ethylene biosynthesis and signaling networks. Plant Cell 14, S131–S151.
Wasternack, C., and Hause, B. (2002). Jasmonates and octadecanoids: signals in plant stress responses and development. Prog. Nucleic Acid Res. Mol. Biol. 72, 165–221.
Xiong, L., Schumaker, K.S., and Zhu, J.-K. (2002). Cell signaling during cold, drought, and salt stress. Plant Cell 14, S165–S183.
Yashuda, M., Ishikawa, A., Jikumaru, Y., Seki, M., Umezawa, T., Asami, T., Maruyama-Nakashita, A., Kudo, T., Shinozaki, K., Yoshida, S., et al. (2008). Antagonistic interaction between systemic acquired resistance and the abscisic acid-mediated abiotic stress response in Arabidopsis. Plant Cell 20, 1678–1692.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Jung, C., Shim, J.S., Seo, J.S. et al. Non-specific phytohormonal induction of AtMYB44 and suppression of jasmonate-responsive gene activation in Arabidopsis thaliana. Mol Cells 29, 71–76 (2010). https://doi.org/10.1007/s10059-010-0009-z
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10059-010-0009-z