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Microarray-based screening of jasmonate-responsive genes in Arabidopsis thaliana

  • Genetics and Genomics
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Abstract

Jasmonates comprise a family of plant hormones that regulate gene expression to modulate diverse developmental and defensive processes. To screen a set of jasmonate-responsive Arabidopsis genes, we performed a microarray analysis using an Affymetrix GeneChip® containing about 8,300 gene probes synthesized in situ. External treatment with 100 μM methyl jasmonate resulted in significant changes (more than twofold increases or decreases) in the expression levels of 137 genes in the rosette leaves of 5-week-old Arabidopsis plants. Of these, 74 genes were up-regulated, including those involved in jasmonate biosynthesis, defense responses, oxidative stress responses, senescence, and cell wall modification. In contrast, the expression of genes involved in chlorophyll constitution and photosynthesis was down-regulated. Most importantly, the jasmonate treatment significantly reduced transcripts of abscisic acid-responsive cold/drought-stress genes, which suggests that an antagonistic interaction occurs between the jasmonate and abscisic acid signaling pathways in abiotic stress responses. Northern blot analysis of some selected genes revealed that the jasmonate-responsive genes exhibited unique time-course expression patterns after the external jasmonate treatment. Based on the basic clustering of the genes, we established a likely regulation scenario: the genes induced early after treatment are involved in signaling mechanisms that activate or repress other genes, whereas intermediate- and late-accumulating genes are activated by the signaling mechanisms and are subsequently involved in the ultimate jasmonate-modulated cellular responses.

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Abbreviations

ABA:

Abscisic acid

AGI:

Arabidopsis genome initiative

cRNA:

Complementary RNA

JA:

Jasmonic acid

MeJA:

Methyl jasmonate

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Acknowledgments

This work was supported by grants from the Crop Functional Genomics Center funded by the Korea Ministry of Science and Technology, and from the Korea Research Foundation (KRF-2004-005-F00013). We also acknowledge support of graduate fellowships from the Ministry of Education through the Brain Korea 21 Project.

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Authors and Affiliations

  1. School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul, 151-921, Korea

    Choonkyun Jung, Seoung Hyun Lyou, SongYion Yeu, Myeong Ae Kim, Sangkee Rhee, Minkyun Kim, Yang Do Choi & Jong-Joo Cheong

  2. School of Biological Sciences, Seoul National University, Seoul, 151-742, Korea

    Jong Seob Lee

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  1. Choonkyun Jung
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  2. Seoung Hyun Lyou
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Correspondence to Jong-Joo Cheong.

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Communicated by J.R. Liu.

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Jung, C., Lyou, S.H., Yeu, S. et al. Microarray-based screening of jasmonate-responsive genes in Arabidopsis thaliana . Plant Cell Rep 26, 1053–1063 (2007). https://doi.org/10.1007/s00299-007-0311-1

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