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Over-expression of TaMYB33 encoding a novel wheat MYB transcription factor increases salt and drought tolerance in Arabidopsis

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Abstract

Salt and drought stresses often adversely affect plant growth and productivity, MYB transcription factors have been shown to participate in the response to these stresses. Here we identified a new R2R3-type MYB transcription factor gene TaMYB33 from wheat (Triticum aestivum). TaMYB33 was induced by NaCl, PEG and ABA treatments, and its promoter sequence contains putative ABRE, MYB and other abiotic stress related cis-elements. Ectopic over-expression of TaMYB33 in Arabidopsis thaliana remarkably enhanced its tolerance to drought and NaCl stresses, but not to LiCl and KCl treatments. The expressions of AtP5CS and AtZAT12 which mirror the activities of proline and ascorbate peroxidase synthesis respectively were induced in TaMYB33 over-expression lines, indicating TaMYB33 promotes the ability for osmotic pressure balance-reconstruction and reactive oxidative species (ROS) scavenging. The up-regulation of AtAAO3 along with down-regulation of AtABF3, AtABI1 in TaMYB33 over-expression lines indicated that ABA synthesis was elevated while its signaling was restricted. These results suggest that TaMYB33 enhances salt and drought tolerance partially through superior ability for osmotic balance reconstruction and ROS detoxification.

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Acknowledgments

This work was supported by the funds of the National Transgenic Project (Grants 2009ZX08009-082B); National Basic Research 973 Program of China (2009CB118300), Major Program of the Natural Science Foundation of China (No. 31030053), the National Science Foundation for Distinguished Young Scholars of China (No. 31000710) and the Encouraging Foundation for the Scientific Research of the Excellent Young and Middle-aged Scientists in Shandong Province (No. BS2011NY001).

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Authors and Affiliations

  1. Department of Biological Science and Biotechnology, University of Jinan, Jinan, 250022, People’s Republic of China

    Yuxiang Qin, Yanchen Tian, Wenxing He & Lu Han

  2. The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Jinan, 250100, People’s Republic of China

    Mengcheng Wang & Guangmin Xia

  3. College of Life Science, Shandong Agricultural University, Taian, 271018, China

    Yanchen Tian

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  1. Yuxiang Qin
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Correspondence to Yuxiang Qin or Guangmin Xia.

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Yuxiang Qin and Mengcheng Wang contributed equally to the work.

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Qin, Y., Wang, M., Tian, Y. et al. Over-expression of TaMYB33 encoding a novel wheat MYB transcription factor increases salt and drought tolerance in Arabidopsis . Mol Biol Rep 39, 7183–7192 (2012). https://doi.org/10.1007/s11033-012-1550-y

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