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Identification of QTLs associated with salinity tolerance at late growth stage in barley

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Abstract

Salinity is a major abiotic stress to barley (Hordum vulgare L.) growth and yield. In the current study, quantitative trait loci (QTL) for yield and physiological components at the late growth stage under salt stress and non-stress environments were determined in barley using a double haploid population derived from a cross between CM72 (salt-tolerant) and Gairdner (salt-sensitive). A total of 30 QTLs for 10 traits, including tiller numbers (TN), plant height, spikes per line (SPL), spikes per plant (SPP), dry weight per plant, grains per plant, grain yield, shoot Na+ (NA) and K+ concentraitions (K) in shoot, and Na+/K+ ratio (NAK), were detected, with 17 and 13 QTLs under non-stress and salt stress, respectively. The phenotypic variation explained by individual QTL ranged from 3.25 to 29.81%. QTL flanked by markers bPb-1278 and bPb-8437 on chromosomes 4H was associated with TN, SPL, and SPP under salt stress. This locus may be useful in the breeding program of marker-assisted selection for improving salt tolerance of barley. However, QTLs associated with NA, K, and NAK differed greatly between non-stress and salt stress environments. It may be suggested that only the QTLs detected under salt stress are really associated with salt tolerance in barley.

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Abbreviations

DH:

Doubled haploid

DWP:

Dry weight per plant

GNP:

Grains per plant

GY:

Grain yield

MAS:

Marker-assisted selection

PH:

Plant height

QTL:

Quantitative trait loci

SPL:

Spikes per line

SPP:

Spikes per plant

TN:

Tillers per plant

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Acknowledgments

We are deeply grateful to Chinese Natural Science Foundation (30630047, 30828023) and Foundation of Zhejiang Educational Committee (20070214) for its financial support. Thanks to Dr Jian Yang for his assistance for map construction and QTL analysis.

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Authors and Affiliations

  1. Department of Agronomy, Zhejiang University, Huajiachi Campus, 310029, Hangzhou, China

    Dawei Xue, Youzong Huang, Kang Wei & Guoping Zhang

  2. Western Australia State Agricultural Biotechnology Centre, Murdoch University, Perth, TAS, Australia

    Xiaoqi Zhang

  3. Department of Agriculture, 3 Baron-Hay Court, South Perth, WA, 6151, Australia

    Sharon Westcott, Chengdao Li & Reg Lance

  4. College of Life and Environment Sciences, Hangzhou Normal University, 310036, Hangzhou, China

    Dawei Xue

  5. College of Agronomy, Henan University of Science and Technology, 471003, Luoyang, China

    Mingcan Chen

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  1. Dawei Xue
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  2. Youzong Huang
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  3. Xiaoqi Zhang
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  4. Kang Wei
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  6. Chengdao Li
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  7. Mingcan Chen
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  8. Guoping Zhang
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  9. Reg Lance
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Correspondence to Guoping Zhang.

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D. Xue and Y. Huang contributed equally to the article.

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Xue, D., Huang, Y., Zhang, X. et al. Identification of QTLs associated with salinity tolerance at late growth stage in barley. Euphytica 169, 187–196 (2009). https://doi.org/10.1007/s10681-009-9919-2

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