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Insights on gene expression response of a characterized resistant genotype of Solanum commersonii Dun. against Ralstonia solanacearum

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Abstract

Solanum commersonii is a wild species related to the cultivated potato. Some S. commersonii genotypes have been proven to be resistant to the pathogenic bacteria Ralstonia solanacearum, which causes damage in potato and other economically important crops. Here an expression analysis of the response of a resistant S. commersonii genotype against R. solanacearum was performed using microarrays. The aims of this work were to elucidate the molecular processes involved in the interaction, establish the timing of the response, and contribute to identify genes related to the resistance. The response to the treatment was already initiated at 6 h post-inoculation (hpi) and was established at 24 hpi; during this period, a high number of genes was differentially expressed and several candidate genes for the resistance of S. commersonii to R. solanacearum were identified. At an early stage, the photosynthetic process was highly repressed and several genes encoding proteins related to reactive oxygen species (ROS) production were differentially expressed. The induction of ERF and ACC-oxidase genes related to the ethylene pathway and PR1 related to the salicylic acid pathway suggested the induction of both pathways, and back up the previously reported hemibiotrophic nature of the pathogen. Five genes related to plant defence and observed to be differentially expressed at the first two time points were validated by real time PCR. This work gives a glimpse to the molecular processes involved in S. commersonii resistance and identifies the species as a valuable genetic source for potato breeding against bacterial wilt.

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Acknowledgments

We would like to thank María Inés Siri, Analía Sanabria and Maria Julia Pianzzola who provided us with the virulent strain UY036 of bacteria. We wish to thank Silvia Restrepo who helped us in the design of the experiment, and Glenn Bryan, Pete Hedley, Salomé Prat and Roland Schafleitner for the invaluable advices and support in the result analysis. The project was financed by FAO (International treaty on plant genetic resources for food and agriculture). This research was carried out in the context of a CYTED program. Rafael Narancio was financed by a Master assistantship of the “Agencia Nacional de Investigación e Innovación (ANII) of Uruguay”.

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Authors and Affiliations

  1. Instituto Nacional de Investigación Agropecuaria, INIA Las Brujas, Ruta 48 km 10, Rincón del Colorado, Uruguay

    R. Narancio, F. Vilaró & M. Dalla Rizza

  2. Unidad de Biología Molecular, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo, Uruguay

    P. Zorrilla & C. Robello

  3. Instituto Nacional de Investigación Agropecuaria, INIA Salto Grande, Salto, Uruguay

    M. Gonzalez

  4. Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Garzón 780, Montevideo, Uruguay

    C. Pritsch

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  1. R. Narancio
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  2. P. Zorrilla
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  3. C. Robello
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  4. M. Gonzalez
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Correspondence to R. Narancio.

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Online resource 1

List of up and down-regulated genes at 6 h post inoculation (hpi). Only genes showing a significant 2-fold change and p-values lower than 0.05 were represented. NA not annotated genes (XLSX 93 kb)

Online resource 2

List of up and down-regulated genes at 24 h post inoculation (hpi). Only genes showing a significant 2-fold change and p-values lower than 0.05 were represented. NA not annotated genes (XLSX 29 kb)

Online resource 3

List of up and down-regulated genes at 48 h post inoculation (hpi). Only genes showing a significant 2-fold change and p-values lower than 0.05 were represented. NA not anotated genes (XLSX 151 kb)

Online resource 4

List of up and down-regulated genes at 120 h post inoculation (hpi). Only genes showing a significant 2-fold change and p-values lower than 0.05 were represented. NA not annotated genes (XLSX 16 kb)

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Narancio, R., Zorrilla, P., Robello, C. et al. Insights on gene expression response of a characterized resistant genotype of Solanum commersonii Dun. against Ralstonia solanacearum . Eur J Plant Pathol 136, 823–835 (2013). https://doi.org/10.1007/s10658-013-0210-y

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