Abstract
PR-1 has been extensively used as a marker for salicylic acid (SA)-mediated defence and systemic and local acquired resistance. The Arabidopsis Genome Project annotates At2g19990 as PR-1. This gene is also identified as PR-1 in two “full genome” Arabidopsis microarrays, and TAIR cites approximately 60 articles to describe its patterns of expression. However, most of these citations are incorrect; the probes used were not At2g19990, but a homologous gene At2g14610, which is annotated as “PR-1-like”. Because of the potential for confusion, we analyzed the expression of both genes in Arabidopsis thaliana (L.) Heynh. At2g14610 (PR-1-like) showed the archetypal patterns of SA-responsive expression: mRNA levels increased following SA-treatment, inoculation with an avirulent (but not a virulent) strain of Pseudomonas syringae, and in wild-type (but not NahG) Arabidopsis infected with cauliflower mosaic virus (CaMV). In cpr5 mutants it was expressed constitutively. In contrast, expression of At2g19990 (annotated as PR-1) was detectable in neither SA-treated Col-0 nor in cpr5. Infection by virulent and avirulent isolates of P. syringae up-regulated expression, but to a similar level, and infection by CaMV induced a modest increase in expression in both the wild type and NahG. At2g19990, although pathogen responsive, does not show the SA-dependent patterns of expression expected from a member of the PR-1 regulon, and its annotation as “PR-1” is inappropriate. The annotations should identify At2g14610 as the authentic PR-1.
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Abbreviations
- CaMV:
-
Cauliflower mosaic virus
- PR:
-
Pathogenesis related
- SA:
-
Salicylic acid
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Acknowledgements
J.L. and V.L. were supported by BBSRC grant 17/P12855 (to J.J.M.). P.A. was supported by BBSRC grant 17/P17237 (to Anna Amtmann, University of Glasgow). P.G. was the recipient of a postgraduate studentship from the Italian Ministry of Education, University and Research (MIUR). This work was funded in part by these BBSRC grants and partly by the Bower Fire Recovery Fund. We also thank Maliha Naeem and Eirini Pachaki for assistance with the blots.
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Laird, J., Armengaud, P., Giuntini, P. et al. Inappropriate annotation of a key defence marker in Arabidopsis: will the real PR-1 please stand up?. Planta 219, 1089–1092 (2004). https://doi.org/10.1007/s00425-004-1355-x
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DOI: https://doi.org/10.1007/s00425-004-1355-x