Abstract
Bacterial wilt of potatoes, which used to be a widespread disease in tropics and subtropics, has become a threat to potato production in temperate region. The diploid species Solanum commersonii has several desirable characteristics including cold tolerance and resistance to several diseases. Selected somatic hybrids between S. tuberosum dihaploid and bacterial-wilt-resistant S. commersonii clones were cross pollinated with S. tuberosum cultivars for further selection of bacterial wilt resistance. The chromosome numbers of the fusion parents were confirmed as 24, and the three fusion hybrids crossed were all tetraploids. The chromosome number of 11 backcross 1 progenies (BC1) was 48 and that of the other six was close to the tetraploid number. Backcross 2 progenies (BC2) were obtained from only three of the 44 BC1 clones crossed. The S. commersonii parent clone, LZ3.2, was the most resistant to bacterial wilt among wild species clones tested. The first sexual progenies segregated for resistance, with one clone highly susceptible and four clones highly resistant. Three highly resistant BC1 clones, CT02-4, CT08-4, and CT10b-4, were backcrossed to cultivars. Two cross combinations produced mostly susceptible BC2 progenies; however seven clones were resistant or highly resistant for both race 1 and race 3. The highly resistant three clones, CT204-3, CT206-9 and CT206-10, were selected for the further testing as cultivars or breeding materials.
Resumen
La marchitez bacteriana de la papa, que ha sido hasta hoy una enfermedad ampliamente distribuida en las regiones tropical y subtropical, se ha convertido en una amenaza para la productión de papa en la región templada. La especie diploide Solanum commersonii tiene varias características deseables incluyendo tolerancia al frío y resistencia a varias enfermedades. Híbridos somáticos seleccionados de haploides de S. tuberosum y clones de la especie resistente S. commersonii,fueron polinizados con cultivares de S. tuberosum para una ulterior selection de resistencia a marchitez bacteriana. Se confirmó como 24 el número de fusión de cromosomas de los progenitores, y los tres híbridos de fusión cruzada fueron todos tetraploides. El número de cromosomas de 11 retrocruzas y de una progenie (BC1) fue 48 y la de los otros seis fue muy cerca al número tetraploide. Se obtuvieron dos progenies de la retrocruza (BC2) solamente en tres de los 44 clones avanzados BC1. Entre todos los clones de especies silvestres probadas el clon progenitor LZ3.2 de S. commersonii fue el más resistente a la marchitez bacteriana. Las primeras progenies sexuales segregaron para resistencia con un clon altamente susceptible y cuatro clones altamente resistentes. Tres clones BC1 altamente resistentes CT02-4, CT08-4 y CT10b-4 fueron retrocruzados a cultivares. Dos combinaciones cruzadas produjeron mayormente progenies susceptibles BC2; sin embargo, siete clones fueron resistentes o altamente resistentes a las razas 1 y 3. Los tres clones altamente resistentes CT204-3, CT206-9 y CT206-10 se seleccionaron para pruebas futuras como cultivares o material de mejoramiento.
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Kim-Lee, H., Moon, J.S., Hong, Y.J. et al. Bacterial wilt resistance in the progenies of the fusion hybrids between haploid of potato andSolanum commersonii . Am. J. Pot Res 82, 129–137 (2005). https://doi.org/10.1007/BF02853650
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DOI: https://doi.org/10.1007/BF02853650