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Transforming growth factor-beta 1 in adipose derived stem cells conditioned medium is a dominant paracrine mediator determines hyaluronic acid and collagen expression profile

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Abstract

Conditioned medium from adipose derived stem cells (ADSC-CM) stimulates both collagen synthesis and migration of fibroblasts, and accelerates wound healing in vivo. Recently, the production and secretion of growth factors has been identified as an essential function of adipose-derived stem cells (ADSCs). However, the main soluble factor of ADSC-CM which mediates paracrine effects and its underlying mechanism has not been elucidated yet. In this study, we considered transforming growth factor-beta1 (TGF-β1) as a strong candidate for paracrine effect of ADSC-CM and investigated collagen synthesis and hyaluronic acid synthase (HAS) expression. After ADSC-CM addition, collagen type I, type III, HAS and hyaluronic acid (HA) expressions on human dermal fibroblasts (HDFs) were evaluated. Furthermore, to clarify effects of TGF-β1 as a paracrine mediator, TGF-β1 antibody and external supplementary TGF-β1 were treated to HDFs. Collagens type I, type III, HAS-1 and HAS-2 mRNA expressions of HDFs were greatly increased by ADSC-CM treatment, however there was no change in TGF-β1 antibody treated HDFs compared with non-treated control. These results strongly demonstrate that TGF-β1 plays an important role as a paracrine mediator of ECM synthesis. The fact that TGF-β1 contained in ADSC-CM not only accelerates collagen deposition but also increase hyaluronic acid synthesis of HDFs through HAS-1 and HAS-2 expression was also elucidated in this study. Therefore, ADSC-CM shows promise for the treatment of cutaneous wounds and accelerates granulation formation during healing process.

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Authors and Affiliations

  1. Biomaterials Research Center, Cellinbio, 103-1208 DigitalEmpire II, 486, Sindong, Yeongtong-gu, Suwon-si, Gyeonggi-do, 443-734, Korea

    Hana Jung & Dong Hee Lee

  2. Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-752, Korea

    Hak Hee Kim & Jong-Chul Park

  3. Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-ku, Seoul, 130-701, Korea

    Yu-Shik Hwang

  4. Department of Dental Biomaterials Science and Dental Research Institute, College of Dentistry, Seoul National University, 28 Yeonkun-dong, Chongro-ku, Seoul, 110-749, Korea

    Hyeong-Cheol Yang

  5. Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-752, South Korea

    Jong-Chul Park

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  1. Hana Jung
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Correspondence to Jong-Chul Park.

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Jung, H., Kim, H.H., Lee, D.H. et al. Transforming growth factor-beta 1 in adipose derived stem cells conditioned medium is a dominant paracrine mediator determines hyaluronic acid and collagen expression profile. Cytotechnology 63, 57–66 (2011). https://doi.org/10.1007/s10616-010-9327-4

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  • DOI: https://doi.org/10.1007/s10616-010-9327-4

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