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Mapping of QTLs associated with abscisic acid and water stress in wheat

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Biologia Plantarum

Abstract

A segregating F4 population from the cross between drought sensitive (Yecora Rojo) and drought tolerant (Pavon 76) genotypes was made to identify molecular markers linked to a wheat (Triticum aestivum L.) abscisic acid (ABA) content at two water regimes. The parents and 150 F4 lines were evaluated phenotypically for drought tolerance using two irrigation treatments [0.25 and 0.75 m3(H2O) m−2(soil)]. Forty different target region amplification polymorphism (TRAP) primer combinations, 98 different sequence-related amplified polymorphism (SRAP) primer combinations, and 400 simple sequence repeat (SSR) primers were tested for polymorphism among the parental genotypes and the F4 lines. Seven loci in the F4 lines treated with the drought stress were identified. Single quantitative trait loci (QTLs) were located on chromosomes 1B, 2A, 3A, 5D, and 7B and each of them explained from 15 to 31 % of phenotypic variance with a LOD value of 7.2 to 15.7. Five QTLs were located on chromosome 4A and six QTLs on chromosome 5A. In control (well-watered) F4 lines, two QTLs were mapped on chromosome 3B and one QTL on each chromosome 5B and 5D. Statistically the most significant groups of QTLs for the ABA content were identified in the regions of chromosomes 3B, 4A, and 5A mostly near to Barc164, Wmc96, and Trap9 markers. Therefore, these markers linked to QTLs for the drought-induced ABA content can be further used in breeding for drought tolerance in wheat.

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Abbreviations

BSA:

bulk segregant analysis

CIM:

composite interval mapping

LOD:

likelihood ratio

QTL:

quantitative trait locus

RIL:

recombinant inbred line

SSR:

simple sequence repeat

SRAP:

sequence-related amplified polymorphism

TRAP:

target region amplification polymorphism

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Authors and Affiliations

  1. Plant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh, Saudi Arabia

    M. N. Barakat, A. A. Al-Doss, K. A. Moustafa, A. A. Elshafei & A. M. Zakri

  2. Crop Science Department, Faculty of Agriculture, University of Alexandria, P.O. Box 21545, Alexandria, Egypt

    M. N. Barakat

  3. College of Pure Science, King Saud University, P.O. Box 2455, Riyadh, Saudi Arabia

    M. S. Saleh & F. H. Al-Qurainy

  4. Genetic Engineering and Biotechnology Division, National Research Centre, P.O. Box 12622, El-Dokki, Cairo, Egypt

    A. A. Elshafei

Authors
  1. M. N. Barakat
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  2. M. S. Saleh
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  3. A. A. Al-Doss
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  4. K. A. Moustafa
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  5. A. A. Elshafei
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  6. A. M. Zakri
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  7. F. H. Al-Qurainy
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Corresponding author

Correspondence to M. N. Barakat.

Additional information

Acknowledgements: The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding the work through the research group project no. RGPVPP-161.

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Barakat, M.N., Saleh, M.S., Al-Doss, A.A. et al. Mapping of QTLs associated with abscisic acid and water stress in wheat. Biol Plant 59, 291–297 (2015). https://doi.org/10.1007/s10535-015-0499-9

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  • DOI: https://doi.org/10.1007/s10535-015-0499-9

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