Abstract
Objectives
To investigate mycothiol peroxidase (MPx) of Corynebacterium glutamicum that is a novel CysGPx family peroxidase using both the mycoredoxin and thioredoxin reducing systems as proton donors for peroxide detoxification and may be involved in the relief of acid stress.
Results
A Δmpx mutant exhibited significantly decreased resistance to acid stress and markedly increased accumulation of reactive oxygen species (ROS) and protein carbonylation levels in vivo. Over-expression of mpx increased the resistance of C. glutamicum to acid stress by reducing ROS accumulation. The stress-responsive extracytoplasmic function-sigma (ECF-σ) factor, SigH, mediated acid-induced expression of mpx in the wild-type under acid conditions, which in turn directly contributed to tolerance to acid stress.
Conclusion
MPx is essential for combating acid stress by reducing intracellular ROS levels induced by acid stress in C. glutamicum, which adds a new dimension to the general physiological functions of CysGPx.
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Acknowledgments
This work was supported by the National High Technology Research and Development Program of China (863 program, Grant 2013AA102802), National Natural Science Foundation of China (Nos. 31270078 and 31500087), Key Science and Technology Research and Development Program of Shaanxi Province, China (2014K02-12-01) and the Opening Project of State Key Laboratory of Microbial Resource, Institute of Microbiology, Chinese Academy of Sciences (No. SKLMR-20120601).
Supporting Information
Supplementary Table 1—Bacterial strains, plasmids and primers used in this study.
Supplementary Figure 1—Deletion of the mpx gene does not affect bacterial growth under normal conditions without acid stress.
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Tietao Wang and Fen Gao have contributed equally to this work.
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Wang, T., Gao, F., Kang, Y. et al. Mycothiol peroxidase MPx protects Corynebacterium glutamicum against acid stress by scavenging ROS. Biotechnol Lett 38, 1221–1228 (2016). https://doi.org/10.1007/s10529-016-2099-y
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DOI: https://doi.org/10.1007/s10529-016-2099-y