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Ogataea kanchanaburiensis sp. nov. and Ogataea wangdongensis sp. nov., two novel methylotrophic yeast species from phylloplane in Thailand

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Abstract

Three strains (KM03T, KM13 T and KM15) representing two novel methylotrophic yeast species were isolated from the external surface of plant leaves, which were collected from Kanchanaburi province, Thailand, by three-consecutive enrichments in methanol broth. Strain KM03T was isolated from phylloplane of a mango tree (Mangifera indica) and two strains, KM13T and KM15, were obtained from phylloplane of different wine grapes (Vitis vinifera). The sequences of the D1/D2 region of the large subunit (LSU) rRNA gene of the two strains (KM13T and KM15) were identical and differed markedly from that of strain KM03T. In terms of pairwise sequence similarity of the D1/D2 region the closest species to the strains KM13T and KM15 were Candida suzukii (CBS 9253T) and Candida nitratophila (CBS 2027T) but with 2.1 % nucleotide substitutions. Strain KM03T differed from Ogataea wickerhamii (CBS 4307T), its closest relative, by 2.3 % nucleotide substitutions. Phylogenetic analysis based on the D1/D2 sequences placed the three strains in the Ogataea clade. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analyses of the D1/D2 and the internal transcribed spacer (ITS) regions of the nuclear ribosomal RNA gene (nrRNA) operon, the three strains represent two novel Ogataea species although formation of ascospores was not observed. Ogataea kanchanaburiensis sp. nov. is proposed for strain KM03T (=BCC 47626T = NBRC 108603T = CBS 12673T). Two strains, KM13T and KM15, were assigned to Ogataea wangdongensis sp. nov. (type strain KM13T = BCC 42664T = NBRC 107778T = CBS 12674T). GenBank/EMBL/DDBJ accession numbers for the sequences of the D1/D2 and the ITS regions of O. kanchanaburiensis KM03T are AB734090 and AB734093, respectively, of O. wangdongensis KM13T are AB734091 and AB734094, respectively, and of O. wangdongensis KM15 are AB734092 and AB734095, respectively.

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References

  • Altschul SF, Madden TL, Schaffer JZ, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  PubMed  CAS  Google Scholar 

  • Dlauchy D, Tornai-Lehoczki J, Fulop L, Péter G (2003) Pichia (Komatagaella) pseudopastoris sp. nov., a new yeast species from Hungary. Antonie Van Leeuwenhoek 83:327–332

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Fonseca A, Inacio J (2006) Phylloplane yeasts. In: Rosa C, Péter G (eds) Biodiversity and ecophysiology of yeasts. Springer, Germany, pp 263–301

    Chapter  Google Scholar 

  • Glushakova AM, Yurkov AM, Chernov IY (2007) Massive isolation of anamorphous ascomycete yeasts Candida oleophila from plant phyllosphere. Mikrobiologiia 76:799–803

    CAS  Google Scholar 

  • Glushakova AM, Maximova IA, Kachalkin AV, Yurkov AM (2010) Ogataea cecidiorum sp. nov., a methanaol-assimilating yeast isolated from galls on willow leaves. Antonie Van Leeuwenhoek 98:93–101

    Article  PubMed  CAS  Google Scholar 

  • Koowadjanakul N, Jindamorakot S, Yongmanitchai W, Limtong S (2011) Ogataea phyllophila sp. nov., Candida chumphonensis sp. nov. and Candida mattranensis sp. nov., three methylotrophic yeast species from phylloplane in Thailand. Antonie Van Leeuwenhoek 100:207–217

    Article  PubMed  Google Scholar 

  • Kuraishi H, Katayama-Fujimura Y, Sugiyama J, Yokoyama T (1985) Ubiquinone systems in fungi. I. Distribution of ubiquinones in the major families of ascomycetes, basidiomycetes, and deuteromycetes, and their taxonomic implications. Trans Mycol Soc Jpn 26:383–395

    Google Scholar 

  • Kurtzman CP (2005) Description of Komagataella phaffii sp. nov. and the transfer of Pichia pseudopastoris to the methylotrophic yeast genus Komagataella. Int J Syst Evol Microbiol 55:973–976

    Article  PubMed  CAS  Google Scholar 

  • Kurtzman CP (2011a) A new methanol assimilating yeast, Ogataea parapolymorpha, the ascosporic state of Candida parapolymorpha. Antonie Van Leeuwenhoek 100:455–462

    Article  PubMed  CAS  Google Scholar 

  • Kurtzman CP (2011b) Ogataea Y. Yamada, K. Maeda & Mikata. In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts, a taxonomic study, 5th edn. Elsevier, Amsterdam, pp 645–671

  • Kurtzman CP, Robnett CJ (2010) Systematics of methanol assimilating yeasts and neighboring taxa from multigene sequence analysis and the proposal of Peterozyma gen. nov., a new member of the Saccharomycetales. FEMS Yeast Res 10:353–361

    Article  PubMed  CAS  Google Scholar 

  • Lachance M-A, Boekhout T, Scorzetti G, Fell JW, Kurtzman CP (2011) Candida Berkhout. In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts, a taxonomic study, 5th edn. Elsevier, Amsterdam, pp 987–1279

    Chapter  Google Scholar 

  • Landell MF, Billodre R, Ramos JP, Leoncini O, Vainstein MH, Valente P (2010) Candida aechmeae sp. nov. and Candida vrieseae sp. nov., novel yeast species isolated from the phylloplane of bromeliads in Southern Brazil. Int J Syst Evol Microbiol 60:244–248

    Article  PubMed  CAS  Google Scholar 

  • Limtong S, Srisuk N, Yongmanitchai W, Kawasaki H, Yurimoto H, Nakase T, Kato N (2004) Three new thermotolerant methylotrophic yeasts, Candida krabiensis sp. nov., Candida sithepensis sp. nov., and Pichia siamensis sp. nov., isolate in Thailand. J Gen Appl Microbiol 50:119–127

    Article  PubMed  CAS  Google Scholar 

  • Limtong S, Yongmanitchai W, Tun MM, Kawasaki H, Seki T (2007) Kazachstania siamensis sp. nov., an ascomycetous yeast species from forest soil in Thailand. Int J Syst Evol Microbiol 57:419–422

    Article  PubMed  Google Scholar 

  • Limtong S, Srisuk N, Yongmanitchai W, Yurimoto H, Nakase T (2008) Ogataea chonburiensis sp. nov. and Ogataea nakhonphanomensis sp. nov., thermotolerant, methylotrophic yeast species isolated in Thailand, and transfer of Pichia siamensis and Pichia thermomethanolica to the genus Ogataea. Int J Syst Evol Microbiol 58:302–307

    Article  PubMed  CAS  Google Scholar 

  • Mikata K, Yamada Y (1995) Ogataea kodamae, a new combination for a methanol-assimilating yeast species, Pichia kodamae van der Walt et Yarrow. Res Commun Inst Ferment 17:99–101

    Google Scholar 

  • Miller JS, Funk VA, Wagner WL, Barrie F, Hoch PC, Herendeen P (2011) Outcomes of the 2011 botanical nomenclature section at the XVIII International Botanical Congress. PhytoKeys 5:1–3

    Article  PubMed  Google Scholar 

  • Morais PB, Teixeira LCRS, Bowles JM, Lachance M-A, Rosa CA (2004) Ogataea falcaomoraisii sp. nov., a sporogenous methylotrophic yeast from tree exudates. FEMS Yeast Res 5:81–85

    Article  PubMed  CAS  Google Scholar 

  • Nagatsuka Y, Saito S, Sugiyama J (2008) Ogataea neopini sp. nov. and O. corticis sp. nov., with the emendation of the ascomycete yeast genus Ogataea, and transfer of Pichia zsoltii, P. dorogensis, and P. trehaloabstinens to it. J Gen Appl Microbiol 54:353–365

    Article  PubMed  CAS  Google Scholar 

  • Nakase T, Suzuki M (1986) Bullera megalospora, a new species of yeast forming large ballistospores isolated from dead leaves of Oryza sativa, Miscanthus sinensis and Sasa sp. in Japan. J Gen Appl Microbiol 32:225–240

    Article  CAS  Google Scholar 

  • Nakase T, Takashima M, Itoh M, Fungsin B, Potacharoen W, Atthasampunna P, Komagata K (2001) Ballistoconidium-froming yeasts found in the phyllosphere of Thailand. Microbiol Cult Coll 17:23–33

    Google Scholar 

  • Nakase T, Imanishi Y, Ninomiya S, Takashima M (2010) Candida rishirensis sp. nov., a novel methylotrophic anamorphic yeast species isolated from soil on Rishiri island in Japan. J Gen Appl Microbiol 56:169–173

    Article  PubMed  CAS  Google Scholar 

  • Nemecek-Marshall M, MacDonald RC, Franzen JJ, Wojciechowski C, Fall R (1995) Methanol emission from leaves. Plant Physiol 108:1359–1368

    PubMed  CAS  Google Scholar 

  • Péter G, Dlauchy D, Tornai-Lehoczki J, Kurtzman CP (2005) Kuraishia molischiana sp. nov., the teleomorph of Candida molischiana. Antonie Van Leeuwenhoek 88:241–247

    Article  PubMed  Google Scholar 

  • Péter G, Tornai-Lehoczki J, Dlauchy D (2007) Ogataea allantospora sp. nov., an ascomycetous yeast species from phylloplane. Antonie Van Leeuwenhoek 92:443–448

    Article  PubMed  Google Scholar 

  • Péter G, Tornai-Lehoczki J, Dlauchy D (2008) Ogataea nitratroaversa sp. nov., a methylotrophic yeast species from temperate forest habitats. Antonie Van Leeuwenhoek 94:217–222

    Article  PubMed  Google Scholar 

  • Péter G, Tornai-Lehoczki J, Dlauchy D (2009) Ogataea populialbae sp. nov., a yeast species from white poplar. FEMS Yeast Res 9:936–941

    Article  PubMed  Google Scholar 

  • Péter G, Dlauchy D, Tornai-Lehoczki J, Gouliamova D, Kurtzman CP (2011) Ogataea saltuana sp. nov., a novel methanol-assimilating yeast species. Antonie Van Leeuwenhoek 100:375–383

    Article  PubMed  Google Scholar 

  • Phaff HJ, Starmer WT (1987) Yeasts associated with plants, insects and soil. In: Rose AH, Harrison JS (eds) The Yeasts, 2nd edn. Academic, London, pp 123–180

    Google Scholar 

  • Slavikova E, Vadkertiova R, Vranova D (2009) Yeasts colonizing the leaves of fruit trees. Ann Microbiol 59:419–424

    Article  CAS  Google Scholar 

  • Suh S-O, Zhou JJ (2010) Methylotrophic yeasts near Ogataea (Hansenula) polymorpha: a proposal of Ogataea angusta comb. nov. and Candida parapolymorpha sp. nov. FEMS Yeast Res 10:631–638

    PubMed  CAS  Google Scholar 

  • Suh SO, Blackwell M, Kurtzman CP, Lachance M-A (2006) Phylogenetics of Saccharomycetales, the ascomycete yeasts. Mycologia 98:1006–1017

    Article  PubMed  CAS  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  PubMed  CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882

    Article  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic, New York, pp 315–322

    Google Scholar 

  • Yamada Y, Kondo K (1973) Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus, and the yeast-like genera Sporobolomyces and Rhodosporidium. J Gen Appl Microbiol 19:59–77

    Article  CAS  Google Scholar 

  • Yamada Y, Maeda K, Mikata K (1994) The phylogenetic relationships of the hat-shaped ascospore-forming, nitrate-assimilating Pichia species, formerly classified in the genus Hansenula Sydow et Sydow, based on the partial sequences of 18S and 26S ribosomal RNAs (Saccharomycetaceae): the proposal of three new genera, Ogataea, Kuraishia, and Nakazawaea. Biosci Biotechnol Biochem 58:1245–1257

    Article  PubMed  CAS  Google Scholar 

  • Yamada Y, Matsuda M, Maeda K, Mikata K (1995) The phylogenetic relationships of methanol-assimilating yeasts based on the partial sequences of 18S and 26S ribosomal RNAs: the proposal of Komagataella gen. nov. (Saccharomycetaceae). Biosci Biotechnol Biochem 59:439–444

    Article  PubMed  CAS  Google Scholar 

  • Yarrow D (1998) Methods for the isolation, maintenance and identification of yeasts. In: Kurtzman CP, Fell JW (eds) The yeasts, a taxonomic study, 4th edn. Elsevier, Amsterdam, pp 77–100

    Chapter  Google Scholar 

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Acknowledgments

This work was supported by the Kasetsart University Research and Development Institute (KURDI), Kasetsart University, Thailand, the Higher Education Research Promotion and National Research University Project of Thailand and Office of the Higher Education Commission, Thailand. Many thanks go to Mr. Teera Yimyoo and Miss Nampueng Koowadjanakul for their assistance in sample collection and yeasts isolation.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand

    Savitree Limtong & Rungluk Kaewwichian

  2. Center for Advanced Studies in Tropical Natural Resources, National Research University, Kasetsart University, Bangkok, 10900, Thailand

    Savitree Limtong

  3. CBS-KNAW Fungal Biodiversity Center, Uppsalalaan, 3584 CT, Utrecht, The Netherlands

    Marizeth Groenewald

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  1. Savitree Limtong
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  2. Rungluk Kaewwichian
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Correspondence to Savitree Limtong.

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Limtong, S., Kaewwichian, R. & Groenewald, M. Ogataea kanchanaburiensis sp. nov. and Ogataea wangdongensis sp. nov., two novel methylotrophic yeast species from phylloplane in Thailand. Antonie van Leeuwenhoek 103, 551–558 (2013). https://doi.org/10.1007/s10482-012-9837-5

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