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Ogataea phyllophila sp. nov., Candida chumphonensis sp. nov. and Candida mattranensis sp. nov., three methylotrophic yeast species from phylloplane in Thailand

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Abstract

Five strains (LN12, LN14T, LN15T, LN16 and LN17T) representing three novel methylotrophic yeast species were isolated from the external surface of plant leaves by three-consecutive enrichments. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene and the phylogenetic analysis, the five strains were assigned to be one novel Ogataea species and two novel Candida species. Three strains (LN12, LN14T and LN16) represent a single novel species of the genus Ogataea, for which the name Ogataea phyllophila sp. nov. is proposed. The type strain is LN14T (= BCC 42666T = NBRC 107780T = CBS 12095T). Strain LN15T was assigned to be Candida chumphonensis sp. nov. (type strain LN15T = BCC 42667T = NBRC 107781T = CBS 12096T). Strain LN17T represented another novel species of Candida that was named Candida mattranensis sp. nov. (type strain LN17T = BCC 42668T = NBRC 107782T = CBS 12097T).

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References

  • Altschul SF, Madden TL, Schaffer JZ, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSIBLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  PubMed  CAS  Google Scholar 

  • Barnett JA, Payne RW, Yarrow D (2000) Yeasts: characteristics and identification. Cambridge University Press, Cambridge

    Google Scholar 

  • Dlauchy D, Tornai-Lehoczki J, Fulop L, Peter G (2003) Pichia (Komatagaella) pseudopastoris sp. nov., a new yeast species from Hungary. Antonie van Leeuwenhoek 83:327–332

    Article  PubMed  CAS  Google Scholar 

  • Edgar PC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acid Res 32:1792–1797

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Fonseca A, Inacio J (2006) Phylloplane yeasts. In: Rosa C, Peter G (eds) Biodiversity and ecophysiology of yeasts. Springer-Verlag, Berlin, Heidelberg, Germany, pp 263–301

    Chapter  Google Scholar 

  • Glushakova AM, Yurkov AM, Chernov IY (2007) Massive isolation of anamorphous ascomycete yeasts Candida oleophila from plant phyllosphere. Microbiology 76:799–803

    Article  CAS  Google Scholar 

  • Glushakova AM, Maximova IA, Kachalkin AV, Yurkov AM (2010) Ogataea cecidiorum sp. nov., a methanaol-assimilating yeast isolated from galls on willow leaves. Antonie van Leeuwenhoek 98:93–101

    Article  PubMed  CAS  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  PubMed  CAS  Google Scholar 

  • Kuraishi H, Katayama-Fujimura Y, Sugiyama J, Yokoyama T (1985) Ubiquinone systems in fungi. I. Distribution of ubiquinones in the major families of ascomycetes, basidiomycetes, and deuteromycetes, and their taxonomic implications. Trans Mycol Soc Jpn 26:383–395

    Google Scholar 

  • Kurtzman CP (1998) Pichia E. C. Hansen emend Kurtzman. In: Kurtzman CP, Fell JW (eds) The yeasts, a taxonomic study, 4th edn. Elsevier, Amsterdam, pp 273–352

    Chapter  Google Scholar 

  • Kurtzman CP (2005) Description of Komagataella phaffii sp. nov. and the transfer of Pichia pseudopastoris to the methylotrophic yeast genus Komagataella. Int J Syst Evol Microbiol 55:973–976

    Article  PubMed  CAS  Google Scholar 

  • Kurtzman CP, Robnett CJ (1998) Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leewenhoek 73:331–371

    Article  CAS  Google Scholar 

  • Kurtzman CP, Robnett CJ (2010) Systematics of methanol assimilating yeasts and neighboring taxa from multigene sequence analysis and the proposal of Peterozyma gen. nov., a new member of the Saccharomycetales. FEMS Yeast Res 10:353–361

    Article  PubMed  CAS  Google Scholar 

  • Landell MF, Billodre R, Ramos JP, Leoncini O, Vainstein MH, Valente P (2010) Candida aechmeae sp. nov. and Candida vrieseae sp. nov., novel yeast species isolated from the phylloplane of bromeliads in Southern Brazil. Int J Syst Evol Microbiol 60:244–248

    Article  PubMed  CAS  Google Scholar 

  • Limtong S, Srisuk N, Yongmanitchai W, Kawasaki H, Yurimoto H, Nakase T, Kato N (2004) Three new thermotolerant methylotrophic yeasts, Candida krabiensis sp. nov., Candida sithepensis sp. nov., and Pichia siamensis sp. nov., isolate in Thailand. J Gen Appl Microbiol 50:119–127

    Article  PubMed  CAS  Google Scholar 

  • Limtong S, Yongmanitchai W, Tun MM, Kawasaki H, Seki T (2007) Kazachstania siamensis sp. nov., an ascomycetous yeast species from forest soil in Thailand. Int J Syst Evol Microbiol 57:419–422

    Article  PubMed  Google Scholar 

  • Limtong S, Srisuk N, Yongmanitchai W, Yurimoto H, Nakase T (2008) Ogataea chonburiensis sp. nov. and Ogataea nakhonphanomensis sp. nov., thermotolerant, methylotrophic yeast species isolated in Thailand, and transfer of Pichia siamensis and Pichia thermomethanolica to the genus Ogataea. Int J Syst Evol Microbiol 58:302–307

    Article  PubMed  CAS  Google Scholar 

  • Meyer SA, Payne RW, Yarrow D (1998) Candida Berkhout. In: Kurtzman CP, Fell JW (eds) The yeasts, a taxonomic study, 4th edn. Elsevier, Amsterdam, pp 454–573

    Chapter  Google Scholar 

  • Mikata K, Yamada Y (1995) Ogataea kodamae, a new combination for a methanol-assimilating yeast species, Pichia kodamae van der Walt et Yarrow. Res Commun Inst Ferment 17:99–101

    Google Scholar 

  • Morais PB, Teixeira LCRS, Bowles JM, Lachance M-A, Rosa CA (2004) Ogataea falcaomoraisii sp. nov., a sporogenous methylotrophic yeast from tree exudates. FEMS Yeast Res 5:81–85

    Article  PubMed  CAS  Google Scholar 

  • Nagatsuka Y, Saito S, Sugiyama J (2008) Ogataea neopini sp. nov. and O. corticis sp. nov., with the emendation of the ascomycete yeast genus Ogataea, and transfer of Pichia zsoltii, P. dorogensis, and P. trehaloabstinens to it. J Gen Appl Microbiol 54:353–365

    Article  PubMed  CAS  Google Scholar 

  • Nakase T, Suzuki M (1986) Bullera megalospora, a new species of yeast forming large ballistospores isolated from dead leaves of Oryza sativa, Miscanthus sinensis and Sasa sp. in Japan. J Gen Appl Microbiol 32:225–240

    Article  CAS  Google Scholar 

  • Nakase T, Takashima M, Itoh M, Fungsin B, Potacharoen W, Atthasampunna P, Komagata K (2001) Ballistoconidium-froming yeasts found in the phyllosphere of Thailand. Microbiol Cult Coll 17:23–33

    Google Scholar 

  • Nakase T, Imanishi Y, Ninomiya S, Takashima M (2010) Candida rishirensis sp. nov., a novel methylotrophic anamorphic yeast species isolated from soil on Rishiri Island in Japan. J Gen Appl Microbiol 56:169–173

    Article  PubMed  CAS  Google Scholar 

  • Peter G, Tornai-Lehoczki J, Fulop L, Dlauchy D (2003) Six new methanol assimilating yeast species from wood material. Antonie van Leeuwenhoek 84:147–159

    Article  PubMed  CAS  Google Scholar 

  • Peter G, Dlauchy D, Tornai-Lehoczki J, Kurtzman CP (2005) Kuraishia molischiana sp. nov., the teleomorph of Candida molischiana. Antonie van Leeuwenhoek 88:241–247

    Article  PubMed  CAS  Google Scholar 

  • Peter G, Tornai-Lehoczki J, Dlauchy D (2007) Ogataea allantospora sp. nov., an ascomycetous yeast species from phylloplane. Antonie van Leeuwenhoek 92:443–448

    Article  PubMed  CAS  Google Scholar 

  • Peter G, Tornai-Lehoczki J, Dlauchy D (2008) Ogataea nitratroaversa sp. nov., a methylotrophic yeast species from temperate forest habitats. Antonie van Leewenhoek 94:217–222

    Article  CAS  Google Scholar 

  • Peter G, Tornai-Lehoczki J, Dlauchy D (2009) Ogataea populialbae sp. nov., a yeast species from white poplar. FEMS Yeast Res 9:936–941

    Article  PubMed  CAS  Google Scholar 

  • Peter G, Tornai-Lehoczki J, Dlauchy D (2010) Ogataea pignaliae sp. nov., the teleomorph of Candida pignaliae. Int J Syst Evol Microbiol 60:2496–2500

    Article  PubMed  Google Scholar 

  • Phaff HJ, Starmer WT (1987) Yeasts associated with plants, insects and soil. In: Rose AH, Harrison JS (eds) The yeasts, 2nd edn. Academic, London, pp 123–180

    Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  • Slavikova E, Vadkertiova R, Vranova D (2009) Yeasts colonizing the leaves of fruit trees. Ann Microbiol 59:419–424

    Article  CAS  Google Scholar 

  • Suh S-O, Zhou JJ (2010) Methylotrophic yeasts near Ogataea (Hansenula) polymorpha: a proposal of Ogataea angusta comb. nov. and Candida parapolymorpha sp. nov. FEMS Yeast Res 10:631–638

    PubMed  CAS  Google Scholar 

  • Suh SO, Blackwell M, Kurtzman CP, Lachance M-A (2006) Phylogenetics of Saccharomycetales, the ascomycete yeasts. Mycologia 98:1006–1017

    Article  PubMed  CAS  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular evolutionary genetics (MEGA) soft-ware version 4.0. Mol Biol Evol 24:1596–1599

    Article  PubMed  CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882

    Article  Google Scholar 

  • Yamada Y, Kondo K (1973) Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus, and the yeast-like genera Sporobolomyces and Rhodosporidium. J Gen Appl Microbiol 19:59–77

    Article  CAS  Google Scholar 

  • Yamada Y, Maeda K, Mikata K (1994) The phylogenetic relationships of the hat-shaped ascospore-forming, nitrate-assimilating Pichia species, formerly classified in the genus Hansenula Sydow et Sydow, based on the partial sequences of 18S and 26S ribosomal RNAs (Saccharomycetaceae): the proposal of three new genera, Ogataea, Kuraishia, and Nakazawaea. Biosci Biotechnol Biochem 58:1245–1257

    Article  PubMed  CAS  Google Scholar 

  • Yamada Y, Matsuda M, Maeda K, Mikata K (1995) The phylogenetic relationships of methanol-assimilating yeasts based on the partial sequences of 18S and 26S ribosomal RNAs: the proposal of Komagataella gen. nov. (Saccharomycetaceae). Biosci Biotechnol Biochem 59:439–444

    Article  PubMed  CAS  Google Scholar 

  • Yarrow D (1998) Methods for the isolation, maintenance and identification of yeasts. In: Kurtzman CP, Fell JW (eds) The yeasts, a taxonomic study, 4th edn. Elsevier, Amsterdam, pp 77–100

    Chapter  Google Scholar 

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Acknowledgments

This work was partially supported by the Thailand Graduate Institute of Science and Technology (TGIST) grant TGIST 01-52-030. The authors would like to thank Assoc Prof Dr Hiroya Yurimoto, Laboratory of Microbial Biotechnology, Graduate School of Agriculture, Kyoto University for allowing to use the microscope for taking yeast micrographs.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand

    Nampueng Koowadjanakul, Wichien Yongmanitchai & Savitree Limtong

  2. Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani, Thailand

    Sasitorn Jindamorakot

  3. Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University, Kasetsart University, Bangkok, 10900, Thailand

    Savitree Limtong

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  1. Nampueng Koowadjanakul
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  2. Sasitorn Jindamorakot
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Correspondence to Savitree Limtong.

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Koowadjanakul, N., Jindamorakot, S., Yongmanitchai, W. et al. Ogataea phyllophila sp. nov., Candida chumphonensis sp. nov. and Candida mattranensis sp. nov., three methylotrophic yeast species from phylloplane in Thailand. Antonie van Leeuwenhoek 100, 207–217 (2011). https://doi.org/10.1007/s10482-011-9579-9

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