Abstract
Following a two-step enrichment in methanol containing broth, methylotrophic yeast strains were isolated from about 45% of the leaf samples collected from broad leafed deciduous trees and from herbs in Hungary. During the enrichment process protists predating the yeasts were observed. Based on standard phenotypical tests and the D1/D2 domain sequences of the large subunit (26S) rDNA of the yeast strains recovered from the phylloplane, some of them represent previously unknown species. The description of a new methylotrophic yeast species, Ogataea allantospora [type strain: NCAIM Y.01822T (CBS 10576, NRRL Y-48267)], isolated from phylloplane is given. The proposed new species is the first member of the genus which forms allantoid ascospores, therefore the emendation of the diagnosis of the genus Ogataea Yamada, Maeda & Mikata is proposed.
Similar content being viewed by others
References
Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402
Barnett JA, Payne RW, Yarrow D (2000) Yeasts: Characteristics and identification, 3rd edn. Cambridge Univ. Press, Cambridge
Dlauchy D, Tornai-Lehoczki J, Fülöp L, Péter G (2003) Pichia (Komagataella) pseudopastoris sp. nov., a new yeast species from Hungary. Antonie van Leeuwenhoek 83:327–332
Fall R, Benson AA (1996) Leaf Methanol – the Simplest Natural Product from Plants. Trends Plant Sci 1:296–301
Fonseca Á, Inácio J (2006) Phylloplane Yeasts. In: Rosa CA, Péter G (eds) Biodiversity and Ecophysiology of Yeasts. Springer Verlag, Berlin, Heidelberg, pp 263–301
Kurtzman CP, Fell JW (eds) (1998) The Yeasts: A Taxonomic Study 4th edn. Elsevier Science Publ., Amsterdam
Kurtzman CP, Robnett CJ (1998) Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73:331–371
Nemecek-Marshall M, MacDonald RC, Franzen JJ, Wojciechowsky CL, Fall R (1995) Methanol Emission from Leaves. Plant Physiol 108:1359–1368
Phaff HJ, Starmer WT (1987) Yeasts Associated with Plants, Insects and Soil. In: Rose AH, Harrison JS (eds) The Yeasts, 2nd edn., Vol 1. Biology of Yeasts. Academic Press, London, pp 123–180
Péter G, Tornai-Lehoczki J, Fülöp L, Dlauchy D (2003) Six new methanol assimilating yeast species from wood material. Antonie van Leeuwenhoek 84:147–159
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882
Yamada Y, Maeda K, Mikata K (1994) The phylogenetic relationships of the hat-shaped ascospore-forming, nitrate-assimilating Pichia species, formerly classified in the genus Hansenula Sydow et Sydow, based on the partial sequences of 18S and 26S ribosomal RNAs (Saccharomycetaceae): the proposals of three new genera, Ogataea, Kuraishia, and Nakazawaea. Biosci Biotech Biochem 58:1245–1257
Yarrow D (1998) Methods for the isolation, maintenance and identification of yeasts. In: Kurtzman CP, Fell JW (eds) The Yeasts, A Taxonomic Study, 4th edn. Elsevier Science Publ., Amsterdam, pp 77–100
Acknowledgements
We thank Alfred Botha (University of Stellenbosch, Department of Microbiology) for sharing the information on the method of the suppression of protists.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Péter, G., Tornai-Lehoczki, J. & Dlauchy, D. Ogataea allantospora sp. nov., an ascomycetous yeast species from phylloplane. Antonie van Leeuwenhoek 92, 443–448 (2007). https://doi.org/10.1007/s10482-007-9172-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10482-007-9172-4