Abstract
Ten strains of a new endophytic ascospore-forming, methanol-assimilating yeast were isolated from the galls induced by sawflies on the leaves of willows in the Losiny Ostrov National Park (Moscow region). Standard phenotypical tests and phylogenetic analyses of 18S rRNA gene, 5.8S-ITS gene region and 26S rRNA gene (D1/D2 domains) sequences showed that the species belongs to the genus Ogataea. We describe it as Ogataea cecidiorum and designate type culture KBP Y-3846 (= CBS 11522T = VKM Y-2982T = VKPM Y-3482T = MUCL 52544T = NCAIM Y.01965T) as the type strain. The new species was registered in MycoBank under MB 515233.
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Acknowledgements
The authors are grateful to Chernov, I.Yu. (Moscow State University, Russia) and Daniel, H.-M. (BCCM/MUCL, Belgium) for their valuable suggestions on the manuscript. We also thank Prokhorov, V. P. (Moscow State University, Russia) for correcting the Latin diagnosis of the novel species. Schäfer, A. M. and Maier, W. (Ruhr-Universität Bochum, Germany) are acknowledged for various assistance. This work was supported by the Russian Foundation for Basic Research, project no. 07-04-00481, and the Russian Federation President’s Program to support the Russian Ph.D. research scientists, project no. MK-5278.2008.4. Andrey Yurkov holds a Post-doc fellowship (A/07/94549) from German Academic Exchange Service (DAAD, Germany). We thank Kurtzman C.P. and Robnett C.J. for sharing unpublished data and suggestions to the manuscript. The type strains of Ogataea nitratoaversa and Pichia trehalophila were kindly provided by Péter G (NCAIM, Hungary) and Daniel H.-M. (BCCM/MUCL, Belgium).
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Fig. S1
PCR fingerprinting profiles with primer M13 for selected strains of O. cecidiorum sp. nov. Lanes: M, markers; N, negative control; 1, ex type of O. cecidiorum; other analysed strains of O. cecidiorum: 2, iG-P2; 3, iG-P3; 4, iG-P4; 5, iG-P5; 6, iG-P6; 7, iG-P7; 8, iG-P8; 9, iG-P9; 10, iG-10. Strains conspecific according to 5.8S-ITS sequence data are marked with asterisk. (TIFF 478 kb)
Fig. S2
Phylogenetic placement of O. cecidiorum obtained by maximum likelihood analysis of 18S rRNA and 26S rRNA (D1/D2 domains) genes. The numbers given on branches are frequencies (>50%) with which a given branch appeared in 1,000 bootstrap replications. The scale indicates the number of expected substitutions accumulated per site. The tree is rooted with Schizosaccharomyces pombe (EU011742 U40085). Sequence accession numbers of type strains are listed, sequences determined in this study are given in bold. (EPS 512 kb)
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Glushakova, A.M., Maximova, I.A., Kachalkin, A.V. et al. Ogataea cecidiorum sp. nov., a methanol-assimilating yeast isolated from galls on willow leaves. Antonie van Leeuwenhoek 98, 93–101 (2010). https://doi.org/10.1007/s10482-010-9433-5
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DOI: https://doi.org/10.1007/s10482-010-9433-5