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Ogataea saltuana sp. nov., a novel methanol-assimilating yeast species

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Abstract

Four ascosporulating strains of an undescribed methanol-assimilating yeast species were isolated from forest habitats in Hungary. Three were recovered from rotten wood and one from leaves of a sessile oak (Quercus petraea). An additional isolate of the undescribed species sharing similar phenotypic characters with the above-noted strains was recovered from the gut of an unidentified beetle collected from under the bark of a coniferous tree in Bulgaria. A closely related, but somewhat divergent strain was recovered from insect frass in a Ponderosa pine (Pinus ponderosa) collected in New Mexico, USA. Analysis of the D1/D2 sequences of the LSU rRNA gene placed the new species in the Ogataea clade. The ITS and the D1/D2 LSU sequences of the rRNA gene repeats were compared for the above-noted strains and that of the type strain of Ogataea zsoltii, the closest neighbour among currently recognized Ogataea species. Their relatedness was investigated by parsimony network analysis as well. As a result of the sequence analysis, it was concluded that the six strains isolated from tree associated habitats represent a single new yeast species. Ogataea saltuana sp. nov. is proposed to accommodate these strains. The type strain NCAIM Y.01833T (CBS 10795T, NRRL Y-48448T) was recovered from rotten wood of Scotch pine (Pinus silvestris) in Hungary. The GenBank accession number for the D1/D2 domain nuclear large subunit rRNA gene sequence of strain NCAIM Y.01833T (CBS 10795T, NRRL Y-48448T) is EU327033. The MycoBank number of the new species is MB 519966.

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Acknowledgment

This work was partly supported by TAMOP 4.2.1/B-09/1/KMR-2010-0005 (D. Dlauchy).

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Authors and Affiliations

  1. National Collection of Agricultural and Industrial Microorganisms, Faculty of Food Sciences, Corvinus University of Budapest, Somlói út 14-16, 1118, Budapest, Hungary

    Gábor Péter, Dénes Dlauchy & Judit Tornai-Lehoczki

  2. Institute of Microbiology, Bulgarian Academy of Sciences, Acad. Bonchev 26, Sofia, 1113, Bulgaria

    Dilnora Gouliamova

  3. Bacterial Foodborne Pathogens and Mycology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL, 61604, USA

    Cletus P. Kurtzman

Authors
  1. Gábor Péter
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  2. Dénes Dlauchy
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  3. Judit Tornai-Lehoczki
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  4. Dilnora Gouliamova
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  5. Cletus P. Kurtzman
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Correspondence to Gábor Péter.

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Péter, G., Dlauchy, D., Tornai-Lehoczki, J. et al. Ogataea saltuana sp. nov., a novel methanol-assimilating yeast species. Antonie van Leeuwenhoek 100, 375–383 (2011). https://doi.org/10.1007/s10482-011-9592-z

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  • DOI: https://doi.org/10.1007/s10482-011-9592-z

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