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Lipid production by Rhodosporidium toruloides Y4 using different substrate feeding strategies

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Journal of Industrial Microbiology & Biotechnology

Abstract

Microbial lipid is a potential alternative feedstock for the biodiesel industry. New culture strategies remain to be developed to improve the economics of microbial lipid technology. This work describes lipid production by the oleaginous yeast Rhodosporidium toruloides Y4 using a 15-l bioreactor with different substrate feeding strategies. Among these strategies, the intermittent feeding mode gave a lipid productivity of 0.36 g l−1 h−1, whereas the constant glucose concentration II (CC-II) mode gave the highest lipid productivity of 0.57 g l−1 h−1. The repeated fed-batch mode according to the CC-II mode was performed with a duration time of 358 h, and the overall lipid productivity was 0.55 g l−1 h−1. Our results suggested that substrate feeding modes had a great impact on lipid productivity and that the repeated fed-batch process was the most appealing method by which to enhance microbial lipid production.

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Acknowledgments

Financial support provided by the National High Technology Research and Development Program of China (2007AA05Z403) and the Knowledge Innovation Program of CAS (KGCX2-YW-336) are gratefully acknowledged.

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Authors and Affiliations

  1. Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, People’s Republic of China

    Xin Zhao, Cuimin Hu, Siguo Wu, Hongwei Shen & Zongbao K. Zhao

  2. Dalian National Laboratory for Clean Energy, Dalian, 116023, People’s Republic of China

    Hongwei Shen & Zongbao K. Zhao

  3. Graduate University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Xin Zhao, Cuimin Hu & Siguo Wu

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  1. Xin Zhao
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  2. Cuimin Hu
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  3. Siguo Wu
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  4. Hongwei Shen
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  5. Zongbao K. Zhao
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Correspondence to Zongbao K. Zhao.

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Zhao, X., Hu, C., Wu, S. et al. Lipid production by Rhodosporidium toruloides Y4 using different substrate feeding strategies. J Ind Microbiol Biotechnol 38, 627–632 (2011). https://doi.org/10.1007/s10295-010-0808-4

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  • DOI: https://doi.org/10.1007/s10295-010-0808-4

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