Summary
Fed-batch culture ofRhodotorula glutinis, an oleaginous yeast, was carried out to obtain high biomass concentration. With air, final concentration of biomass was 100–110 g/L, whereas with oxygen-enriched air, 185 g/L was obtained. Obligate aerobic metabolism ofR. glutinis under oxygen limitation and low oxygen requirement under lipidaccumulating condition were found to be advantageous for the high density culture of this microbe.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Andreevskaya, V. D., and Zalashko, M. V. (1979). Prik. Biokhim. Mikrobiol., 15, 522.
AOAC (1980) in “Official Methods of Analysis of the AOAC”, 13th ed., pp. 858.
Choi, S. Y., Ryu, D. D. Y., and Rhee, J. S. (1982) Biotechnol. Bioeng. 24, 1165.
Davies, J. and Gordon, T. (1984). in “Proceedings of 6th Australian Biotechnology Conference,” pp. 127, Univ. of Queensland, St. Lucia, Brisbane.
Folch, J., Lees, M., and Sloane-Stanley, G. H. (1957). J. Biol. Chem. 226, 497.
Gill, C. O., Hall, M. J., and Ratledge, C. (1977). Appl. Env. Microbiol., 33, 231.
Matsumura, M. (1983). Ferment. Ind. (in Japanese), 41, 102.
Mori, H., Yano, T., Kobayashi, T., and Shimizu, S. (1979). J. Chem. Eng. Japan., 12, 313.
Pan, J. G., and Rhee, J. S. (1985a). Biotechnol. Bioeng., 28, 112.
Pan, J. G., and Rhee, J. S. (1985b). Korean J. Chem. Eng., 2, 81.
Pan, J. G., Rhee, J. S. and Lebeault, J. M. (1986). Biotechnol. Lett., Submitted.
Ratledge, C. (1982). Prog Ind. Microbiol., 16, 119.
Rattray, J. B. M. (1984). JAOCS, 61, 1701.
Yoon, S. H., and Rhee, J. S. (1983). JAOCS, 60, 1281.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pan, J.G., Kwak, M.Y. & Rhee, J.S. High density cell culture ofRhodotorula glutinis using oxygen-enriched air. Biotechnol Lett 8, 715–718 (1986). https://doi.org/10.1007/BF01032568
Issue Date:
DOI: https://doi.org/10.1007/BF01032568