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A Genome Scan for Quantitative Trait Loci Associated with Vibrio anguillarum Infection Resistance in Japanese Flounder (Paralichthys olivaceus) by Bulked Segregant Analysis

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Abstract

A recent genetic linkage map was employed to detect quantitative trait loci (QTLs) associated with Vibrio anguillarum resistance in Japanese flounder. An F1 family established and challenged with V. anguillarum in 2009 was used for QTL mapping. Of the 221 simple sequence repeat (SSR) markers used to detect polymorphisms in the parents of F1, 170 were confirmed to be polymorphic. The average distance between the markers was 10.6 cM. Equal amounts of genomic DNA from 15 fry that died early and from 15 survivors were pooled separately to constitute susceptible bulk and resistance bulk DNA. Bulked segregant analysis and QTL mapping were combined to detect candidate SSR markers and regions associated with the disease. A genome scan identified four polymorphic SSR markers, two of which were significantly different between susceptible and resistance bulk (P = 0.008). These two markers were located in linkage group (LG) 7; therefore, all the SSR markers in LG7 were genotyped in all the challenged fry by single marker analysis. Using two different models, 11–17 SSR markers were detected with different levels of significance. To confirm the associations of these markers with the disease, composite interval mapping was employed to genotype all the challenged individuals. One and three QTLs, which explained more than 60 % of the phenotypic variance, were detected by the two models. Two of the QTLs were located at 48.6 cM. The common QTL may therefore be a major candidate region for disease resistance against V. anguillarum infection.

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Acknowledgements

The work was supported by grants from State 863 High-Technology R&D Project (2012AA10A408), the National Major Basic Research Program (2010CB126303) of China, the Taishan Scholar Project of Shandong Province, the Special Fund for Agro-Scientific Research in the Public Interest of China (200903046), and the National Natural Sciences Foundation of China (30871918).

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Authors and Affiliations

  1. College of Marine Life Science, Ocean University of China, 266003, Qingdao, The People’s Republic of China

    Lei Wang

  2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, 266071, Qingdao, The People’s Republic of China

    Lei Wang, Caixia Fan, Yang Liu, Yingping Zhang, Han Deng, Ying Xu, Yongsheng Tian, Xiaolin Liao, Mingshu Xie, Wenlong Li & Songlin Chen

  3. College of Fisheries, Henan Normal University, 453007, Xinxiang, The People’s Republic of China

    Lei Wang

  4. Haiyang Yellow Sea Aquatic Product Co., Ltd, 265122, Yantai, The People’s Republic of China

    Shoutang Liu & Deqiang Sun

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  1. Lei Wang
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Correspondence to Songlin Chen.

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Wang, L., Fan, C., Liu, Y. et al. A Genome Scan for Quantitative Trait Loci Associated with Vibrio anguillarum Infection Resistance in Japanese Flounder (Paralichthys olivaceus) by Bulked Segregant Analysis. Mar Biotechnol 16, 513–521 (2014). https://doi.org/10.1007/s10126-014-9569-9

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