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Major Histocompatibility Complex Class IIB Allele Polymorphism and Its Association with Resistance/Susceptibility to Vibrio anguillarum in Japanese Flounder (Paralichthys olivaceus)

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Abstract

The full length of major histocompatibility complex (MHC) class IIB cDNA was cloned from a Chinese population of Paralichthys olivaceus by homology cloning and rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR). The MHC IIB genomic sequence is 1,864 bp long and consists of 34-bp 5′UTR, 741-bp open reading frame, 407-bp 3′UTR, 96-bp intron1, 392-bp intron2, 85-bp intron3, and 109-bp intron4. Phylogenetic analysis showed that the putative MHC class IIB amino acid of the Chinese P. olivaceus shared 28.3% to 85.4% identity with that of the reported MHC class IIB in other species. A significant association between MHC IIB polymorphism and disease resistance/susceptibility was found in Chinese P. olivaceus. Thirteen different MHC IIB alleles were identified among 411 clones from 84 individuals. Among the 280 (268) nucleotides, 32 (11.4%) nucleotide positions were variable. Most alleles such as alleles a, b, c, d, e, f, j, k, i, m were commonly found in both resistant and susceptible stock. Via χ2 test, allele d was significantly more prevalent in individuals from susceptible stock than from resistant stock, and their percentages were 23.80% and 7.14%, respectively. In addition, allele g occurred in 9 and allele h in 4 of 42 resistant individuals that were not present in the susceptible stock; their percentages were 21.4% and 9.52%, respectively. Although allele l was found only in 8 individuals from the susceptible stock, its percentage is 19.05%.

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Acknowledgments

This work was supported by grants from National Major Basic Research Program (973) (2004CB117403) and National Nature Science Foundation of China (30413240) and state 863 High-Technology R&D Project of China (2002AA626010).

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Authors and Affiliations

  1. Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 266071, Qingdao, People's Republic of China

    Y. X. Zhang, S. L. Chen, Y. G. Liu & Z. X. Sha

  2. College of Marine Life Science, Ocean University of China, 266003, Qindao, People's Republic of China

    Y. X. Zhang

  3. The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquaculture, Auburn University, Auburn, AL, 36849, USA

    Z. J. Liu

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  1. Y. X. Zhang
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  2. S. L. Chen
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Correspondence to S. L. Chen.

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Zhang, Y.X., Chen, S.L., Liu, Y.G. et al. Major Histocompatibility Complex Class IIB Allele Polymorphism and Its Association with Resistance/Susceptibility to Vibrio anguillarum in Japanese Flounder (Paralichthys olivaceus). Mar Biotechnol 8, 600–610 (2006). https://doi.org/10.1007/s10126-005-6185-8

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