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Genetic Mapping and QTL Analysis of Growth-Related Traits in the Pacific Oyster

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Abstract

The Pacific oyster (Crassostrea gigas) is one of the most important oysters cultured worldwide. To analyze the oyster genome and dissect growth-related traits, we constructed a sex-averaged linkage map by combining 64 genomic simple sequence repeats, 42 expressed sequence tag-derived SSRs, and 320 amplified fragment length polymorphism markers in an F1 full-sib family. A total of 426 markers were assigned to 11 linkage groups, spanning 558.2 cM with an average interval of 1.3 cM and 94.7% of genome coverage. Segregation distortion was significant for 18.8% of the markers (P < 0.05), and distorted markers tended to occur on some genetic regions or linkage groups. Most growth-related quantitative traits were highly significantly (P < 0.01) correlated, and principal component analysis obtained four principal components. Quantitative trait locus (QTL) analysis identified three significant QTLs for two principal components, which explained 0.6–13.9% of the phenotypic variation. One QTL for sex was detected on linkage group 6, and the inheritabilities of sex for parental alleles and maternal alleles on that locus C15 are 39.8% and 0.01%, respectively. The constructed linkage map and determined QTLs can provide a tool for further genetic analysis of the traits and be potential for marker-assisted selection in C. gigas breeding.

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Acknowledgments

This work was supported by grants from the 973 Program (2010CB126406) and the National Marine Public Welfare Research Program (200905020).

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Authors and Affiliations

  1. College of Fisheries, Ocean University of China, Qingdao, 266003, China

    Xiang Guo, Qi Li, Qing Z. Wang & Ling F. Kong

Authors
  1. Xiang Guo
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  2. Qi Li
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  3. Qing Z. Wang
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  4. Ling F. Kong
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Corresponding author

Correspondence to Qi Li.

Electronic Supplementary Material

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Table S1

AFLP primer combinations information (DOC 88 kb)

Table S2

A comparison of the characteristics of the linkage maps constructed for C. gigas (DOC 85 kb)

Table S3

Loadings of the separated traits on the principal component (n = 83) in F1 progeny (DOC 81 kb)

Table S4

A comparison of the female and male quantitative traits (DOC 82 kb)

Fig. S1

Quantile–quantile plot test for normal distribution of principal components. PC principal component (DOC 334 kb)

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Guo, X., Li, Q., Wang, Q.Z. et al. Genetic Mapping and QTL Analysis of Growth-Related Traits in the Pacific Oyster. Mar Biotechnol 14, 218–226 (2012). https://doi.org/10.1007/s10126-011-9405-4

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  • DOI: https://doi.org/10.1007/s10126-011-9405-4

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