Abstract
Microalgae are important biological resources which attract the researchers for the production of valuable products like bio-energy and value-added products. In this study, enhanced accumulation of carotenoids and lipids was obtained from Coelastrella sp. M60 which was grown at 40 °C, along with different stress conditions (nitrogen starvation, salinity and TiO2). The results clearly indicate that osmotic stress and nitrogen starvation along with high temperature had induced the yield of zeaxanthin (13.15 mg/g DCW) and the lipid content (39.51 ± 5.13%). Further, it was also observed that TiO2 combined with other stresses had induced the increased accumulation of astaxanthin and zeaxanthin up to 1.16 and 0.51 fold on the 10th day of cultivation. On the contrary, TiO2, along with other stress conditions had influenced the biomass production up to 1.16 fold than the control, but no detectable increase in lipid content was observed. Thus, for the first time it is reported that by employing combined stress factors such as high temperature, nitrogen starvation and 3% salinity had resulted in significant improvement in zeaxanthin production in the foresaid microalgae. Further, it was also noticed that the Coelastrella sp. M60 had accumulated the carotenoids and lipids under TiO2 along with other oxidative stress conditions within the short duration.
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Authors thankfully acknowledge DST-PURSE, DST-FIST, UGC-SAP, UGC-UPE and Central Instrumentation facility of Madurai Kamaraj University, Madurai, for providing the instrumentation facilities.
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Pushpalatha, S., Sangeetha, R., Ariraman, S. et al. Photocatalyst (TiO2) as an enhancer: an attempt to enhance the production of carotenoids and lipids with the combined oxidative stresses in Coelastrella sp. M60. Clean Techn Environ Policy 23, 41–53 (2021). https://doi.org/10.1007/s10098-020-01879-y
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DOI: https://doi.org/10.1007/s10098-020-01879-y