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Medium optimization using mathematical statistics for production of β-Carotene by Blakeslea trispora and fermenting process regulation

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Abstract

Optimization of medium components for enhancement of β-carotene production by Blakeslea trispora was achieved using mathematical statistics. Optimum concentrations of components using Plackett-Burman design and response surface methodology (RSM) were d-glucose 7.16%, wheat bran extract 4.08%, MgSO4 0.04%, soybean oil 3%, thiamine 0.01%, soybean meal 1%, and KH2PO4 0.2%. Maximum production of β-carotene using optimized medium was 643 mg/L in a shake flask. A predicted value of 669 mg/L was based on results of an RSM regression. The optimum aeration rate of 1.5 vvm produced 866 mg/L and the optimum agitation speed of 100 rpm produced 975 mg/L of β-carotene. The t of a quadratic model using regression derived coefficients was significant. Maximum production of β-carotene using the optimized medium in a stirred tank reactor (10 L) at an optimal aeration rate and an optimum agitation speed with addition of 0.1%(v/v) β-ionone was 1,357 mg/L.

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References

  1. Paz ED, Ángel Martín, Estrella A. Soraya Rodríguez-Rojo, Matias A A. Formulation of β-carotene by precipitation from pressurized ethylacetate-on-water emulsions for application as natural colorant. Foodhyd. 26: 17–27 (2012)

    Google Scholar 

  2. Papaioannou EH, Stoforos NG, Liakopoulou-Kyriakides M. Substrate contribution on free radical scavenging capacity of carotenoid extracts produced from Blakeslea trispora cultures. World J. Microbiol. Biotechnol. 27: 851–858 (2011)

    Article  CAS  Google Scholar 

  3. Kim JH, Kim SW, Nguyen DQA, Li He, Kim SB, Seo YG, Yang JK, Chung IY, Kim DH, Kim CJ. Production of β-carotene by recombinant E. coli with engineered whole mevalonate pathway in batch and fed-batch cultures. Biotechnol. Bioprocess Eng. 14: 559–564 (2009)

    Article  CAS  Google Scholar 

  4. Li Z, Ma XQ, Li A, Zhang CW. A novel potential source of β-carotene: Eustigmatoscf polyphem (Eustigmatophyceae) and pilot β-carotene production in bubble column and flat panel photobioreactors. Bioresour. Technol. 117: 257–263 (2012).

    Article  CAS  Google Scholar 

  5. Bhosale P. Environmental and cultural stimulants in the production of carotenoids from microorganisms. Appl. Microbiol. Biotechnol. 63: 351–361 (2004)

    Article  CAS  Google Scholar 

  6. Cvetković D, Marković D. Beta-carotene suppression of benzophenone-sensitized lipid peroxidation in hexane through additional chain-breaking activities. Radiat. Phys. Chem. 80: 76–84 (2011)

    Article  Google Scholar 

  7. Ribeiro BD, Barreto DW, Coelho MAZ. Technological aspects of acarotene production. Food Bioprocess Technol. 5: 693–701 (2011)

    Article  Google Scholar 

  8. Lan CH, Fougère R, Waché Y. Increase in stability and change in supramolecular structure of β-carotene through encapsulation into polylactic acid nanoparticles. Food Chem. 124: 42–49 (2011)

    Article  CAS  Google Scholar 

  9. Ribeiro BD, Coelho MAZ, Barreto DW. Production of concentrated natural beta-carotene from buriti (Mauritia vinifera) oil by enzymatic hydrolysis. Food Bioprod. Process 2: 141–147 (2011)

    Google Scholar 

  10. Mantzouridou F, Roukas T, Kotzekidou P. Effect of the areration rate and agitation speed on β-carotene production and morphology of Blakslea trispora in a stirred tank reactor:mathematical modeling. Biochem. Eng. J. 10: 123–135 (2002)

    Article  CAS  Google Scholar 

  11. Varzakakou M, Roukas T, Kotzekidou P. Effect of the ratio of (+) and (-) mating type of Blakeslea trispora on carotene production from cheese whey in submerged fermentation. World J. Microbiol. Biotechnol. 12: 2151–2156 (2010)

    Article  Google Scholar 

  12. Yan GL, Wen KR, Duan CQ. Enhancement of β-carotene production by over-expression of HMG-CoA reductase coupled with addition of ergosterol biosynthesis inhibitors in recombinant Saccharomyces cerevisiae. Curr. Microbiol. 64: 159–163 (2012)

    Article  CAS  Google Scholar 

  13. Hanchinal VM, Survase SA, Sawant SK, Annapure US. Response surface methodology in media optimization for production of β-carotene from Daucus carota. Plant Cell Tiss. Organ. Cult. 93: 123–132 (2008)

    Article  CAS  Google Scholar 

  14. Kuzina V, Ramírez-Medina H, Visser H, Cerdá-Olmedo E. Genes involved in carotenesynthesis and mating in Blakeslea trispora. Curr. Genet. 54: 143–152 (2008).

    Article  CAS  Google Scholar 

  15. Mehta BJ, Obraztsova IN, Cerdá-Olmedo E. Mutants and intersexual heterokaryons of Blakeslea trispora for production of β-carotene and lycopene. Appl. Environ. Microbiol. 7: 4038–4043 (2003)

    Google Scholar 

  16. Roukas T, Niavi P, Kotzekidou P. A new medium for spore production of Blakeslea trispora using response surface methodology. World J. Microb. Biot. 27: 307–317 (2011)

    Article  CAS  Google Scholar 

  17. Goksungur Y, Mantzouridou F, Roukas T, Kotzekidou P. Production of β-carotene from beet molasses by Blakeslea trispora in stirredtank and bubble column reactors. Appl. Biochem. Biotechnol. 112: 37–54 (2004)

    Article  CAS  Google Scholar 

  18. Sheetal C, Singhal R. Media optimization for the production of β-carotene by Blakslea trispora: A statistical approach. Bioresour. Technol. 99: 722–730 (2008)

    Article  Google Scholar 

  19. Mohammad R, Nasri N, Seyed Hadi R. Optimization of β-carotene production by a mutant of the lactose-positive yeast Rhodotorula acheniorum from whey ultrafiltrate. Food Sci. Biotechnol. 20: 445–454 (2011)

    Article  Google Scholar 

  20. Mantzouridou F, Roukas T, Kotzekidoua P. Optimization of β-carotene production from synthetic medium by Blakeslea trispora a mathematical modeling. Appl. Biochem. Biotechnol. 101:153–175 (2002)

    Article  CAS  Google Scholar 

  21. Xu F, Yuan QP, Zhu Y. Improved production of lycopene and β-carotene by Blakeslea trispora with oxygen-vectors. Process Biochem. 42: 289–293 (2007)

    Article  CAS  Google Scholar 

  22. Nanou K, Roukas T, Kotzekidou P. Role of hydrolytic enzymes and oxidative stress in autolysis and morphology of Blakeslea trispora during β-carotene production in submerged fermentation. Appl. Microbiol. Biotechnol. 74: 447–453 (2007)

    Article  CAS  Google Scholar 

  23. Mantzouridou F, Roukas T, Achatz B. Effect of oxygen transfer rate on β-carotene production from synthetic medium by Blakeslea trispora in shake flask culture. Enzyme Microb. Technol. 37: 687–694 (2005)

    Article  CAS  Google Scholar 

  24. Sheetal MC, Ananthanarayan L, Rekha SS. Use of metabolic stimulators and inhibitors for enhanced production of β-carotene and lycopene by Blakeslea trispora NRRL 2895 and 2896. Bioresour. Technol. 99: 3166–3173 (2008)

    Article  Google Scholar 

  25. Nanou K, Roukas T. Stimulation of the biosynthesis of carotenes by oxidative stress in Blakeslea trispora induced by elevated dissolved oxygen levels in the culture medium. Bioresour. Technol. 102: 8159–8164 (2011)

    Article  CAS  Google Scholar 

  26. Nanou K, Roukas T, Papadakis E. Oxidative stress and morphological changes in Blakeslea trispora induced by enhanced aeration during carotene production in a bubble column reactor. Biochem. Eng. J. 54: 172–177 (2011)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan, 430068, China

    Zuowen Yan, Changgao Wang, Jianguo Lin & Jun Cai

  2. College of Bioengineering, Hubei University of Technology, Wuhan, 430068, China

    Zuowen Yan, Changgao Wang, Jianguo Lin & Jun Cai

Authors
  1. Zuowen Yan
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  2. Changgao Wang
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  3. Jianguo Lin
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  4. Jun Cai
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Correspondence to Jun Cai.

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Yan, Z., Wang, C., Lin, J. et al. Medium optimization using mathematical statistics for production of β-Carotene by Blakeslea trispora and fermenting process regulation. Food Sci Biotechnol 22, 1667–1673 (2013). https://doi.org/10.1007/s10068-013-0265-8

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  • DOI: https://doi.org/10.1007/s10068-013-0265-8

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